Automated instrument for timecourse metabolic studies of bacteria. Systeme automatise pour l'etude dynamique du metabolisme bacterien.

用于细菌时程代谢研究的自动化仪器。

• 批准号: 389817-2010
• 负责人: Roy, Sébastien
• 金额: $ 9.22万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443031
• 关键词: Automated; instrument; timecourse; metabolic; studies

The study of bacterial adaptation to environmental change requires rigorous culture conditions. In our laboratories, we achieve this using bioreactors (fermentors) which have the added advantage of allowing the control and monitoring of many physico-chemical parameters, which enable us to produce culture conditions unattainable otherwise. The bacteria we study are of utmost interest in the fields of health (Vibrio cholerae, Staphylococcus aureus), biotechnology (Streptomyces spp.), and environment (the nitrogen-fixing Frankia sp.). Some of these share also the characteristics of sudden dormancy in late-phase cultures and warrant research in to their mechanisms of adaptation to environmental stress. The automated sampling and analysis instrument requested will allow us to expand our research into new horizons, exploiting proteomic and transcriptomic strategies to better understand how horizontal gene transfer translates into ecological fitness in Vibrio cholerae, and how environmental conditions affect the metabolism in Streptomyces spp., S. aureus, and Frankia sp. The instrument requested will allow the continuous sampling and monitoring of nutrients and metabolites in continuous bioreactor cultures which would not be feasible otherwise. It will be central to the training of highly qualified personnel in microbiology and biotechnology.

研究细菌对环境变化的适应性需要严格的培养条件。在我们的实验室中，我们使用生物反应器（发酵罐）实现这一目标，这些生物反应器具有控制和监测许多物理化学参数的额外优势，使我们能够生产其他方法无法达到的培养条件。我们研究的细菌在健康（霍乱弧菌，金黄色葡萄球菌），生物技术（链霉菌属），和环境（固氮的Frankia sp.）。其中一些还具有在后期培养中突然休眠的特征，并值得研究其适应环境胁迫的机制。所要求的自动化采样和分析仪器将使我们能够将研究扩展到新的领域，利用蛋白质组学和转录组学策略更好地了解水平基因转移如何转化为霍乱弧菌的生态适应性，以及环境条件如何影响链霉菌属的代谢，S.所要求的仪器将允许在连续生物反应器培养物中连续取样和监测营养物和代谢物，否则这是不可行的。它将是培养微生物学和生物技术方面高素质人员的中心。