Instrument Development: A lab-scale soft X-ray microscope for biological systems

仪器开发：用于生物系统的实验室规模软 X 射线显微镜

• 批准号: EP/Z53108X/1
• 负责人: Jeremy Frey
• 金额: $ 514.71万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FZ53108X%2F1
• 关键词: Instrument; Development; lab; scale; soft

This instrument development application proposes the construction of a laboratory based, laser driven, water window x-ray microscope, capable of label free, nanometre spatial resolution, of biological samples with minimal sample damage.The system makes use of the coherent nature of the x-ray radiation generated by high harmonic generation (HHG) from intense short pulse laser pulses. The coherent radiation is the basis of lensless imaging using algorithmic phase reconstruction (e.g. Coherent Diffraction Imaging and Ptychography).The project builds on the current 29 nm imaging systems developed at Southampton and using recent advances in reliable fibre based laser technologies and in line with the Rosalind Franklin Institutes aims, will produce a system with more than an order of magnitude increase in both spatial resolution, data acquisition time, and enable the imaging of hydrated samples with minimal sample damage.The system will be tested with several collaborations and in conjunction with the RFI and Harwell Research Complex imaging capabilities, and will be engineered to become a reliable facility to complement existing capabilities at the Central Laser Facility

该仪器开发应用提出了一种基于实验室的、激光驱动的、水窗X射线显微镜的构造，该显微镜能够以最小的样品损伤对生物样品进行无标记、纳米空间分辨率的分析。该系统利用了由强短脉冲激光脉冲产生的高次谐波（HHG）产生的X射线辐射的相干性质。相干辐射是利用算法相位重建实现无透镜成像的基础该项目建立在目前在南安普顿开发的29纳米成像系统的基础上，并使用可靠的基于光纤的激光技术的最新进展，并符合罗莎琳德富兰克林研究所的目标，将产生一个在空间分辨率，该系统将与RFI和Harwell Research Complex的成像能力一起进行测试，并将被设计成一个可靠的设施，以补充中央激光设施的现有能力