Fluorescence spectrophotometer for biophysical analysis

用于生物物理分析的荧光分光光度计

• 批准号: 389993-2010
• 负责人: Paetzel, Mark
• 金额: $ 5.55万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443079
• 关键词: Fluorescence; spectrophotometer; biophysical; analysis

We request a fluorescence spectrophotometer that will be of great resource to a broad range of biochemistry laboratories at SFU. This new instrument will relieve pressure from an older model overburdened Cary instrument found in the Chemistry Department and make possible experiments currently outside of the current instrument's sensitivity, temperature range and speed of data acquisition. We will be using this instrument to: perform enzyme kinetics studies on antibiotic and antiviral targets (Paetzel Lab); to evaluate cardiac troponin c protein interaction (Tibbits Lab); to investigate the ability of intact double helical DNAs to recognize homology under conditions of molecular crowding (Sen Lab); to characterize RNA aptamers against dyes that become strongly florescent upon rigidification (Unrau Lab); to characterize the protein complexes involved in apoptosis and cancer (Pio Lab); and to test for conformational changes in receptor proteins (Young Lab). In addition, many other research labs in various Departments at SFU will have access to the instrument (MBB, Chemistry, Biology, Kinesiology, Faculty of Health Sciences) under the supervision and training of experienced spectroscopists. The sensitivity, accuracy and speed of data acquisition from this new instrument will be a great benefit to laboratories at SFU and will play an important role in the development of new antibiotics, new antivial drugs, new medications and treatments for congenital heart disease, cancer and many other diseases, as well as facilitating the development of real time biological tracking methodologies and nanotechnology devices. Our research fields and Canada in general will benefit greatly from the acquisition of this instrument, it will make possible experiments that will elucidate the kinetics, dynamics, and structure of medically and scientifically important biological macromolecules.

我们需要一台荧光分光光度计，这将是SFU广泛的生物化学实验室的重要资源。这台新仪器将减轻化学系旧型号的超负荷Cary仪器的压力，并使目前超出现有仪器灵敏度、温度范围和数据采集速度的实验成为可能。我们将使用该仪器：进行抗生素和抗病毒靶点的酶动力学研究（Paetzel实验室）；评估心肌肌钙蛋白c蛋白相互作用（Tibbits实验室）；研究完整的双螺旋dna在分子拥挤条件下识别同源性的能力（Sen实验室）；表征RNA适体对固化后产生强烈荧光的染料的抗性（Unrau实验室）；表征参与细胞凋亡和癌症的蛋白复合物（Pio实验室）；并测试受体蛋白的构象变化（Young实验室）。此外，SFU各个部门的许多其他研究实验室（MBB，化学，生物，运动机能学，健康科学学院）将在经验丰富的光谱专家的监督和培训下使用该仪器。从这台新仪器获取数据的灵敏度、准确性和速度将极大地有利于SFU的实验室，并将在开发新的抗生素、新的抗菌药、新的药物和治疗先天性心脏病、癌症和许多其他疾病的方法，以及促进实时生物跟踪方法和纳米技术设备的发展方面发挥重要作用。我们的研究领域和整个加拿大将从这台仪器的获得中受益匪浅，它将使阐明医学和科学上重要的生物大分子的动力学，动力学和结构的实验成为可能。