Fluorescence spectrophotometer for biophysical analysis

我们要求一个荧光分光光度计，这将是巨大的资源，以广泛的生物化学实验室在SFU。 这种新仪器将减轻压力，从一个旧型号的负担过重的卡里仪器发现在化学系，并使可能的实验目前以外的目前仪器的灵敏度，温度范围和速度的数据采集。 我们将使用该仪器：对抗生素和抗病毒靶点进行酶动力学研究（Paetzel Lab）;评价心肌肌钙蛋白c蛋白相互作用（Tibbits实验室）;研究完整双螺旋DNA在分子拥挤条件下识别同源性的能力（Sen实验室）;表征RNA适体对在硬化后变得强烈荧光化的染料的特性（Unrau Lab）;表征参与细胞凋亡和癌症的蛋白质复合物（Pio Lab）;以及测试受体蛋白质的构象变化（Young Lab）。此外，SFU各部门的许多其他研究实验室将在经验丰富的光谱学家的监督和培训下使用该仪器（MBB，化学，生物学，运动机能学，健康科学学院）。 从这个新仪器的灵敏度，准确性和数据采集速度将是一个很大的好处，在SFU实验室，并将发挥重要作用，在新的抗生素，新的抗病毒药物，新的药物和治疗先天性心脏病，癌症和许多其他疾病的发展，以及促进真实的时间生物跟踪方法和纳米技术设备的发展。 我们的研究领域和加拿大一般将大大受益于该仪器的收购，它将使可能的实验，将阐明动力学，动力学和结构的医学和科学上重要的生物大分子。

We request a fluorescence spectrophotometer that will be of great resource to a broad range of biochemistry laboratories at SFU. This new instrument will relieve pressure from an older model overburdened Cary instrument found in the Chemistry Department and make possible experiments currently outside of the current instrument's sensitivity, temperature range and speed of data acquisition. We will be using this instrument to: perform enzyme kinetics studies on antibiotic and antiviral targets (Paetzel Lab); to evaluate cardiac troponin c protein interaction (Tibbits Lab); to investigate the ability of intact double helical DNAs to recognize homology under conditions of molecular crowding (Sen Lab); to characterize RNA aptamers against dyes that become strongly florescent upon rigidification (Unrau Lab); to characterize the protein complexes involved in apoptosis and cancer (Pio Lab); and to test for conformational changes in receptor proteins (Young Lab). In addition, many other research labs in various Departments at SFU will have access to the instrument (MBB, Chemistry, Biology, Kinesiology, Faculty of Health Sciences) under the supervision and training of experienced spectroscopists. The sensitivity, accuracy and speed of data acquisition from this new instrument will be a great benefit to laboratories at SFU and will play an important role in the development of new antibiotics, new antivial drugs, new medications and treatments for congenital heart disease, cancer and many other diseases, as well as facilitating the development of real time biological tracking methodologies and nanotechnology devices. Our research fields and Canada in general will benefit greatly from the acquisition of this instrument, it will make possible experiments that will elucidate the kinetics, dynamics, and structure of medically and scientifically important biological macromolecules.