Critical Use UVvis Spectrophotometer

关键用途紫外可见分光光度计

• 批准号: RTI-2023-00154
• 负责人: Hudson, Robert
• 金额: $ 3.93万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=747918
• 关键词: Critical; Use; UVvis; Spectrophotometer

This funding request is for the replacement and upgrade of obsolete instrumentation that is well beyond its serviceable lifetime. The Hudson group has used a Varian Cary 300 Bio since 1999 which suffered a catastrophic failure of the Peltier device (3 years ago) after a litany of other serious problems (motherboard and optical train) which were serviced in recent years at no small expense, but is no longer able to be repaired.  As such, we no longer have adequate access to instrumentation to pursue our research. The current instrument has been critical to our research mission for the past 22 years. The requested instrument will primarily service the Hudson group (average group: 4 graduate, 2 UG); Konermann group (average group: 8 graduate, 2 undergraduate) and the Workentin group (average group: 6 graduate, 2 UG). As with our previous instrument, we expect and welcome regular use from the Wisner group (2-3 students, for characterization of photo-switchable molecules), the Luyt group (7-8 students, for quantum yield calculation in conjugation with our PTI spectrofluorimeter) and occasional use from groups. As well, the new instrument with extended red-range useful for gold-nanoclusters and organic and inorganic fluorophores (Gilroy group, 11 students).  The Varian Cary 300 instrument has been superseded by the Agilent Cary 3500 which possesses significantly improved design, by replacement of the error-prone mechanical transport by a fixed eight-position sample chamber that is temperature controlled by a Peltier-device and a multi-beam design. Additionally, the re-designed instrument uses a xenon lamp instead of the dual deuterium/visible lamp design of the Cary 300 which is more robust, economical and is capable of fast data collection for use in studying the kinetics of binding interactions. The instrument also features an air-cooled Peltier heating/cooling block eliminating the need for an external bath, which is also a point of failure eliminated. The instrument has the sensitivity and photometric stability suitable for the long experiments typically needed to study melts and renaturation curves. Additionally, rapid temperature ramping, with in-cell temperature monitoring, is available - this feature will be exploited to develop improved methodologies for determining nucleic acid analog thermal stabilities in a more time-economical fashion.

这项供资请求用于更换和更新远远超过其使用寿命的陈旧仪器。哈德逊小组自1999年以来一直使用Varian卡里300 Bio，在一系列其他严重问题（主板和光学系统）之后，Peltier设备发生灾难性故障（3年前），近年来花费了不小的费用进行维修，但再也无法修复。因此，我们不再有足够的仪器来进行研究。目前的仪器在过去22年中对我们的研究使命至关重要。所要求的仪器将主要服务于哈德逊组（平均组：4名研究生，2名UG）; Konermann组（平均组：8名研究生，2名本科生）和Workentin组（平均组：6名研究生，2名UG）。与我们以前的仪器一样，我们期待并欢迎维斯纳小组（2-3名学生，用于表征光开关分子），Luyt小组（7-8名学生，用于与我们的PTI荧光分光光度计结合的量子产率计算）的定期使用和偶尔使用。此外，新仪器具有扩展的红色范围，适用于金纳米团簇和有机及无机荧光团（Gilroy组，11名学生）。Varian卡里300仪器已被Agilent卡里3500仪器取代，Agilent 3500仪器具有显著改进的设计，通过将容易出错的机械传送替换为由Peltier温度控制的固定八位置样品室，设备和多波束设计。此外，重新设计的仪器使用氙灯，而不是卡里300的双氘/可见灯设计，其更耐用、更经济，并且能够快速收集数据，用于研究结合相互作用的动力学。该仪器还具有一个空气冷却的Peltier加热/冷却块，无需外部浴槽，这也是一个故障点消除。该仪器具有灵敏度和光度稳定性，适用于通常需要研究熔解和复性曲线的长时间实验。此外，快速升温，与细胞内温度监测，是可用的-这一特点将被利用来开发改进的方法，用于确定核酸类似物的热稳定性，在一个更节省时间的方式。