A system for protein purification

蛋白质纯化系统

• 批准号: 390116-2010
• 负责人: Christendat, DineshSingh
• 金额: $ 4.98万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443112
• 关键词: system; protein; purification

The Christendat lab uses structural, enzymological and biochemical approaches to investigate enzymes of the shikimate pathway in plants and microbes. There are two major themes being investigated in the lab: 1) The mechanism by which the pathway enzymes are regulated in plants and 2) the evolutionary relationship of the shikimate pathway enzymes in plants and microbes. The results from these two themes will contribute to a better understanding of the shikimate pahway which could eventually facilitate metabolic engineering of crops to enhance their nutritional contents and also facilitate the development of novel antimicrobial and herbicidal agents. This protein purification system requested would enhance our ability to prepare high quality proteins, which are essential for our research program.

克里斯滕达特实验室使用结构、酶学和生化方法来研究植物和微生物中莽草酸途径的酶。实验室正在研究两个主要主题：1)植物中途径酶的调节机制；2)植物和微生物中莽草酸途径酶的进化关系。这两个主题的结果将有助于更好地理解莽草酸途径，最终可能促进作物的新陈代谢工程，以提高其营养含量，并促进新型抗菌和除草剂的开发。所要求的这种蛋白质纯化系统将增强我们制备高质量蛋白质的能力，这对我们的研究计划至关重要。