Regulation of the shikimate pathways in Arabidopsis

拟南芥中莽草酸途径的调控

• 批准号: 250203-2006
• 负责人: Christendat, DineshSingh
• 金额: $ 3.19万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=394690
• 关键词: Regulation; shikimate; pathways; Arabidopsis

This project focuses on the plant shikimate metabolic pathway.  The shikimate pathway is important for the biosynthesis of the aromatic amino acids, some vitamins, flavonoids, isoflavonoids and phenylpropanoids.  In plants, more than 30 % of fixed carbon flows through the shikimate pathway for the biosynthesis of these aromatic compounds.  These compounds are important for plant defence and environmental responses, in maintaining plant structural integrity and in their growth and development.   Furthermore, the aromatic amino acids are essential in our diet and flavonoids and isoflavonoids have known phyto properties; for example some isoflavonoids serves as phyto-estrogen.  Some of these compounds also have chemo-therapeutic properties.  The shikimate pathway is also found in fungi, microbes and apicomplexan parasites, but not in humans, as such it is actively being studied for the development of novel drugs and herbicides.  This proposal deals with the biochemical characterization and structure determination of the enzymes dehydroquinate dehydratease, shikimate dehydrogenase and shikimate kinase in Arabidopsis thaliana.

本项目的重点是植物莽草酸代谢途径。莽草酸途径对芳香族氨基酸、某些维生素、类黄酮、甘草酸和苯丙素类化合物的生物合成至关重要。在植物中，超过30%的固定碳流经莽草酸途径用于这些芳香族化合物的生物合成。这些化合物对植物防御和环境响应至关重要，维持植物结构的完整性以及它们的生长和发育。 此外，芳香族氨基酸在我们的饮食中是必不可少的，类黄酮和黄酮类化合物具有已知的植物特性;例如，一些植物雌激素。这些化合物中的一些也具有化学治疗特性。莽草酸途径也在真菌、微生物和顶复门寄生虫中发现，但在人类中没有，因此，它正被积极地研究，用于开发新的药物和除草剂。本提案涉及脱氢奎尼酸脱水酶的生物化学表征和结构测定，拟南芥中莽草酸脱氢酶和莽草酸激酶。