A preparative FPLC for biomolecular research

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The aim of this proposal is to acquire preparative fast protein liquid chromatography (FPLC) instrumentation for biological macromolecule purification and characterization in the departments of Physics and Chemistry. FPLC is an essential experimental technique for efficient and scalable purification of proteins, polysaccharides, and other biological macromolecules. This instrumentation is required for a growing number of interdisciplinary research programs in biophysics, bioanalytical chemistry, biomaterials, and chemical biology that require a facile and scalable method of biomolecular purification. The requested FPLC from GE Healthcare is a robust, versatile system that can be used with a wide range of chromatography media to purify biomolecules in the large-scale quantities required for these programs. Beyond its important impact on the specific research programs described in this proposal, acquisition of this FPLC system will enhance interdisciplinary research and training efforts within the Faculty of Science, the Centre for Advanced Research in Environmental Genomics, and the NSERC CREATE training programs in "Quantitative Biomedicine" and "Medicinal Chemistry and Biopharmaceutical Development" by providing an important resource for the training of HQP.