A preparative FPLC for biomolecular research

这项计划的目的是在物理系和化学系获得用于生物大分子纯化和表征的制备快速蛋白质液相色谱(FPLC)仪器。FPLC是高效、可扩展纯化蛋白质、多糖和其他生物大分子的重要实验技术。在生物物理、生物分析化学、生物材料和化学生物学中，越来越多的跨学科研究项目需要这种仪器，这些项目需要一种简单且可扩展的生物分子纯化方法。GE Healthcare要求的FPLC是一个强大的、多功能的系统，可以与各种层析介质一起使用，以提纯这些计划所需的大规模生物分子。除了对本计划中描述的具体研究计划产生重要影响外，收购FPLC系统还将加强理学院、环境基因组学高级研究中心和NSERC内部的跨学科研究和培训努力，通过为HQP培训提供重要资源，创建“定量生物医学”和“药物化学和生物制药开发”培训计划。

The aim of this proposal is to acquire preparative fast protein liquid chromatography (FPLC) instrumentation for biological macromolecule purification and characterization in the departments of Physics and Chemistry. FPLC is an essential experimental technique for efficient and scalable purification of proteins, polysaccharides, and other biological macromolecules. This instrumentation is required for a growing number of interdisciplinary research programs in biophysics, bioanalytical chemistry, biomaterials, and chemical biology that require a facile and scalable method of biomolecular purification. The requested FPLC from GE Healthcare is a robust, versatile system that can be used with a wide range of chromatography media to purify biomolecules in the large-scale quantities required for these programs. Beyond its important impact on the specific research programs described in this proposal, acquisition of this FPLC system will enhance interdisciplinary research and training efforts within the Faculty of Science, the Centre for Advanced Research in Environmental Genomics, and the NSERC CREATE training programs in "Quantitative Biomedicine" and "Medicinal Chemistry and Biopharmaceutical Development" by providing an important resource for the training of HQP.