Fast protein liquid chromatography (FPLC) system for the structural studies of the PHD finger proteins in yeast

用于酵母 PHD 指蛋白结构研究的快速蛋白液相色谱 (FPLC) 系统

• 批准号: 390710-2010
• 负责人: Sheng, Yi
• 金额: $ 5.88万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443269
• 关键词: Fast; protein; liquid; chromatography; FPLC

Funds to purchase a Fast Protein Liquid Chromatography (FPLC) protein purification system are requested to aid the structural and functional studies of the yeast Plant Homeo-Domain (PHD) finger proteins led by the new investigator Dr. Yi Sheng at York University. The PHD finger proteins are small zinc-binding proteins, found to be involved in many important cellular processes such as gene regulation, immunity and development. The requested equipment will be used to purify yeast PHD finger proteins for three-dimensional structure determination and interaction studies with other cellular proteins. The outcome of this research will provide insight into the functional divergence of PHD finger proteins and reveal how mutations in these PHD finger affect cellular processes. The FPLC system is essential to the success of the proposed research.

申请资金购买快速蛋白液相色谱（FPLC）蛋白纯化系统，以帮助约克大学新研究员Yi Sheng博士领导的酵母植物同源结构域（PHD）指蛋白的结构和功能研究。 PHD指蛋白是一类小的锌结合蛋白，参与基因调控、免疫和发育等重要的细胞过程。所要求的设备将用于纯化酵母PHD指蛋白，用于三维结构测定和与其他细胞蛋白的相互作用研究。这项研究的结果将提供深入了解PHD指蛋白的功能分歧，并揭示这些PHD指中的突变如何影响细胞过程。 FPLC系统是所提出的研究的成功至关重要。