Fast protein liquid chromatography (FPLC) system for the structural studies of the PHD finger proteins in yeast

需要资金购买快速蛋白质液相色谱(FPLC)蛋白质纯化系统，以帮助约克大学新任研究员易盛博士领导的酵母植物同源结构域(PHD)指状蛋白质的结构和功能研究。PhD Finger蛋白是一种小的锌结合蛋白，被发现参与了许多重要的细胞过程，如基因调节、免疫和发育。所要求的设备将用于纯化酵母PHD指状蛋白，用于三维结构测定和与其他细胞蛋白的相互作用研究。这项研究的结果将为深入了解PhD指蛋白的功能差异提供洞察，并揭示这些PhD指蛋白的突变如何影响细胞过程。FPLC系统对拟议研究的成功至关重要。

Funds to purchase a Fast Protein Liquid Chromatography (FPLC) protein purification system are requested to aid the structural and functional studies of the yeast Plant Homeo-Domain (PHD) finger proteins led by the new investigator Dr. Yi Sheng at York University. The PHD finger proteins are small zinc-binding proteins, found to be involved in many important cellular processes such as gene regulation, immunity and development. The requested equipment will be used to purify yeast PHD finger proteins for three-dimensional structure determination and interaction studies with other cellular proteins. The outcome of this research will provide insight into the functional divergence of PHD finger proteins and reveal how mutations in these PHD finger affect cellular processes. The FPLC system is essential to the success of the proposed research.