Fast protein liquid chromatography (FPLC) system for the structural studies of the PHD finger proteins in yeast

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Funds to purchase a Fast Protein Liquid Chromatography (FPLC) protein purification system are requested to aid the structural and functional studies of the yeast Plant Homeo-Domain (PHD) finger proteins led by the new investigator Dr. Yi Sheng at York University. The PHD finger proteins are small zinc-binding proteins, found to be involved in many important cellular processes such as gene regulation, immunity and development. The requested equipment will be used to purify yeast PHD finger proteins for three-dimensional structure determination and interaction studies with other cellular proteins. The outcome of this research will provide insight into the functional divergence of PHD finger proteins and reveal how mutations in these PHD finger affect cellular processes. The FPLC system is essential to the success of the proposed research.