Vibratome for immunocytochemistry and single cell elcetrical recordings

用于免疫细胞化学和单细胞电记录的 Vibratome

• 批准号: 390669-2010
• 负责人: NovalesFlamarique, Inigo
• 金额: $ 0.97万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443259
• 关键词: Vibratome; immunocytochemistry; single; cell; elcetrical

Funds are requested to purchase a vibratome for immunocytochemical and electrophysiological studies of retinal cells using fixed and live tissue sections. These studies constitute the body of my research program which aims to characterize the molecular mechanisms regulating visual pigment protein (opsin) expression in vertebrate photoreceptors and the spatio-temporal organization of ganglion cell projections from the retina to the brain. This research is of fundamental importance to understand photoreceptor development in the vertebrate retina and the cellular mechanisms that guide colour and polarization vision.

请拨款购买震动器，用于使用固定和活组织切片对视网膜细胞进行免疫细胞化学和电生理研究。这些研究构成了我的研究项目的主体，旨在表征脊椎动物光感受器中视觉色素蛋白（opsin）表达的分子机制，以及从视网膜到大脑的神经节细胞投射的时空组织。这项研究对于理解脊椎动物视网膜感光器的发育以及指导颜色和偏振视觉的细胞机制具有重要意义。