Request for gradient polymerase chain reaction thermocycler and -86 C Ultra-low freezer

请求梯度聚合酶链式反应热循环仪和-86°C超低温冰箱

• 批准号: 390685-2010
• 负责人: PercivalSmith, Anthony
• 金额: $ 1.68万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443265
• 关键词: Request; gradient; polymerase; chain; reaction

Gradient PCR Thermocycler: Polymerase chain reaction (PCR) is a work horse of molecular biology. Both the Percival-Smith and Kelly lab use PCR for mutagenesis, clone construction, clone detection, genome analysis, RT-PCR, and sequence analysis. Neither lab has access to a gradient thermocycler that allows rapid optimization of annealing temperature for PCR reactions. The machine will be in a room accessible to both

梯度PCR热循环仪：聚合酶链反应（PCR）是分子生物学的一项重要工作。Percival-Smith和Kelly实验室都使用PCR进行诱变、克隆构建、克隆检测、基因组分析、RT-PCR和序列分析。这两个实验室都没有使用梯度热循环仪，可以快速优化PCR反应的退火温度。这台机器将在一个房间，