Elucidating mechanisms of gene restoration

阐明基因恢复机制

• 批准号: 341446-2007
• 负责人: Lolle, Susan
• 金额: $ 2.63万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=458383
• 关键词: Elucidating; mechanisms; gene; restoration

Mendel's laws of inheritance have guided our exploration of classical as well as molecular genetics through much of the last century. According to these laws genetic traits segregate independently and are inherited stably from one generation to the next. Although notable exceptions to the law of stable inheritance exist, all have been relatively well defined. We have recently demonstrated that Arabidopsis plants homozygous for recessive mutant alleles of the HOTHEAD locus can inherit DNA sequence information that is absent from the chromosomal genome of their parents. Our published data suggest that sequences throughout the nuclear genome are susceptible to change and that both coding and non-coding regions are affected. Additional research results demonstrate that deleted sequences can be accurately restored and suggest that the process is template-directed. Not surprisingly, these findings are extremely controversial.

孟德尔的遗传定律在上个世纪的大部分时间里指导着我们对经典遗传学和分子遗传学的探索。 根据这些规律，遗传性状独立分离，并从一代稳定地遗传到下一代。 虽然稳定继承法存在明显的例外，但所有例外都有相对明确的定义。 我们最近已经证明，HOTHEAD基因座的隐性突变等位基因纯合的拟南芥植物可以继承其父母的染色体基因组中不存在的DNA序列信息。 我们发表的数据表明，整个核基因组的序列容易发生变化，编码区和非编码区都受到影响。其他研究结果表明，删除的序列可以准确地恢复，并表明该过程是模板导向的。毫不奇怪，这些发现极具争议。