Evaluation of inactivation efficacy of viral pathogens using integrated biowaste utilization system-pathogen destruction unit (IBUS-p)

使用综合生物废物利用系统-病原体破坏单元（IBUS-p）评估病毒病原体的灭活效果

• 批准号: 396315-2010
• 负责人: Pang, Xiaoli
• 金额: $ 1.82万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=467549
• 关键词: Evaluation; inactivation; efficacy; viral; pathogens

A novel detection method has been developed by integrating virus growth in cultured cells and real time polymerase chain reaction (ICC-rtPCR). This method could spot the viruses in drinking water and wastewater samples, and identify whether the virus is alive and infectious as well. ICC-rtPCR is very sensitive and much better than current cell culture method recommended by US Environment Protect Agency. Using this method, we have found living viruses associated with human and animal diarrhea in the treated wastewater. Undoubtedly, we can promote drinking water safety and public health when this technology is ratified in monitoring drinking water and environmental surface water.

将病毒在培养细胞中的生长与实时聚合酶链反应（ICC-rtPCR）相结合，建立了一种新的检测方法。这种方法可以在饮用水和废水样本中发现病毒，并确定病毒是否活着和传染性。icc -rt - pcr灵敏度高，比目前美国环境保护署推荐的细胞培养方法要好得多。利用这种方法，我们在处理后的废水中发现了与人类和动物腹泻相关的活病毒。毫无疑问，当这项技术被批准用于监测饮用水和环境地表水时，我们可以促进饮用水安全和公众健康。