Molecular mechanisms of catalysis and the basis of substrate specificity in polysaccharide hydrolases

多糖水解酶催化的分子机制和底物特异性的基础

• 批准号: ARC : DP0210955
• 负责人: Dr Hugues Driguez
• 金额: $ 23.1万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2002
• 资助国家: 澳大利亚
• 起止时间: 2002-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/molecular-mechanisms-catalysis-polysaccharide-hydrolases/74627
• 关键词: Molecular; mechanisms; catalysis; basis; substrate

Molecular mechanisms of catalysis and the basis of substrate specificity in polysaccharide hydrolases. Reaction intermediates along hydrolytic pathways and molecular determinants of substrate specificity of barley B-glucan exo- and endohydrolases will be defined using crystallographic and kinetic analyses. These enzymes are of central importance in cell wall metabolism during development of higher plants, and in plant-pathogen interactions. Realization of the project objectives will not only provide fundamental information on catalytic mechanisms, but will also provide opportunities to manipulate enzyme specificity. Further, site-directed mutagenesis of the enzymes will be used to generate glycosynthases, which will be evaluated for their ability to synthesise novel oligosaccharide and polysaccharide products, some of which might show immunomodulating activity.

多糖水解酶催化作用的分子机制和底物特异性的基础。反应中间体沿着水解途径和分子决定因素的底物特异性的大麦B-葡聚糖外切酶和内切水解酶将被定义使用晶体学和动力学分析。这些酶在高等植物发育过程中的细胞壁代谢以及植物-病原体相互作用中具有核心重要性。项目目标的实现不仅将提供关于催化机制的基本信息，而且还将提供操纵酶特异性的机会。此外，酶的定点诱变将用于产生糖苷酶，将评估其合成新的寡糖和多糖产物的能力，其中一些可能显示免疫调节活性。