A microvolume spectrophotometer for quantitating DNA and protein, and measuring cell density

本申请的目的是申请一台UV-VIS分光光度计，这是任何分子生物学和生物化学实验室的基本和必不可少的仪器。这些仪器经常被用来确定DNA和蛋白质样本的浓度，或者细菌细胞培养的密度，这些都是我们实验室使用的许多程序的重要测量。我们目前有权使用西屋大学理学院的两台较老的UV-Vis分光计。然而，出于几个原因，继续依赖这些工具是不可行的。首先，现有的一种仪器的位置使其在常规基础上使用不切实际。第二种仪器的位置更方便；然而，它的使用量很大，因此往往无法获得。此外，我们对现有仪器的依赖随着我的实验室团队的规模而增加，由于威斯康星大学和理工学院生物学研究活动的扩大，两种仪器的压力都在增加。由于它对我的研究计划至关重要，因此我申请资金购买一台UV-Vis分光光度计，放在我的实验室里。这将消除我们对旧工具的依赖，也将缓解这些工具的一些压力，这些工具目前超额认购，变得不太可靠。我已经要了一台NanoDrop ND-2000c，这是一种微型分光光度计。微量分光光度技术消除了将样品转移到试管中的需要，并允许直接测量样品的吸光度，通常无需稀释。因此，可以使用小至0.5微米的L样品，从而节省宝贵的样品和时间，可用于更重要的应用。特别要求使用NanoDrop ND-2000c，因为它还具有使用更传统的基于试管的方法测量吸光度的能力，这是不适用于直接“纳米滴”测量方法的某些样品类型所必需的。该仪器将向威尔弗里德·劳里埃大学理学院的其他研究人员提供。

The purpose of this application is to request a UV-Vis spectrophotometer, which represents a basic and essential instrument for any molecular biology and biochemistry lab. These instruments are used frequently to determine the concentration of DNA and protein samples, or the density of bacterial cell cultures, for example, which are important measurements for many procedures employed in our lab. We currently have access to two older UV-Vis spectrophotometers in the Faculty of Science at WLU. However, continued reliance on these instruments is not feasible for several reasons. First, the location of one of the available instruments makes its use on a regular basis impractical. The second instrument is more conveniently located; however, it is heavily used, and is therefore often unavailable. Furthermore, our reliance on the existing instruments has increased with the size of my lab group, and pressure on both instruments is increasing due to expansion of research activities in Biology at WLU and the Faculty of Science in general. Because of its central importance to my research program, I have therefore requested funds for the acquisition of a UV-Vis spectrophotometer to be housed within my laboratory. This will eliminate our reliance on the older instruments, and will relieve some of the pressure from those instruments as well, which are currently oversubscribed and becoming less realiable. I have requested a NanoDrop ND-2000c, which is a microvolume spectrophotometer. Microvolume spectrophotometry technology eliminates the need to transfer samples to a cuvette, and allows absorbance of the sample to be measured directly usually without dilution. For this reason, sample volumes as small as 0.5 µl can be used, thereby saving valuable sample and time which can be devoted fto more important applications. The NanoDrop ND-2000c is specifically requested, as it also has the capacity to measure absorbance using the more traditional cuvette-based approach, which is required for some sample types that are not amenable to the direct "nanodrop" method of measurement. The instrument will be made available to other researchers in the Faculty of Science at Wilfrid Laurier University.