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Microelectrode puller requested for electrophysiologists at the university of waterloo

滑铁卢大学电生理学家需要微电极拉拔器

基本信息

批准号：
407023-2011
负责人：
Spafford, David
金额：
$ 1.57万
依托单位：
University of Waterloo
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2010
资助国家：
加拿大
起止时间：
2010-01-01 至 2011-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472608
关键词：
Microelectrode puller requested electrophysiologists university

项目摘要

The University of Waterloo Neuroscience Research Group including Dr. David Spafford and Dr. Matthijs van der Meer (Department of Biology) requests a Zeitz Model DMZ Universal Microelectrode Puller produced by Zeitz-Instruments Vertriebs GmbH in Germany. The Zeitz puller is available in North America through Dagan Corporation of Minneapolis, Minnesota for $21,560 CAD. The fabrication of microelectrodes for electrophysiological recording is an essential tool for neurophysiologists. A microelectrode puller is in use every day with research associates, post-doctoral fellows, graduate students and undergraduate students. There is only one micropipette puller at the University of Waterloo. It is a twelve year old, P-97 Flaming/Brown Micropipette Puller that has stopped functioning properly. It is imperative that a second puller be available because there is no other puller available for crafting microelectrodes at the University of Waterloo. We are looking to replace the old micropipette puller with a Zeitz Model DMZ Universal Microelectrode Puller. The Zeitz model is a new generation puller that will pull electrodes from glass and also automatically polish the tips for electrophyiological recording. There are vertical micropipette pullers which make less precise tips (Sutter P-30 / Kopf) and more expensive Laser-Based machines (Sutter P-2000), but neither of these suited to our needs. We include only one quote for the Zeitz Model puller because there is no other equivalent puller on the market.

滑铁卢大学神经科学研究小组包括David Spafford博士和Matthijs van der Meer博士（生物系）要求德国Zeitz- instruments Vertriebs GmbH生产的Zeitz Model DMZ通用微电极拉器。Zeitz牵引器在北美通过明尼苏达州明尼阿波利斯市的达根公司销售，售价为21,560加元。制造用于电生理记录的微电极是神经生理学家必不可少的工具。每天都有研究人员、博士后、研究生和本科生使用微电极拉杆。滑铁卢大学只有一个微移管工。这是一架12岁的P-97火焰/棕色微移液器已经停止正常工作。由于滑铁卢大学没有其他可用于制作微电极的工具，因此必须提供第二个工具。我们希望用Zeitz模型DMZ通用微电极拉器取代旧的微移液管拉器。Zeitz模型是一种新一代的拉器，它可以从玻璃上拉出电极，并自动抛光尖端，以便进行电生理记录。有垂直微移液器，使不太精确的尖端（Sutter P-30 / Kopf）和更昂贵的激光机器（Sutter P-2000），但这些都不适合我们的需要。我们只包括Zeitz型号拉杆的报价，因为市场上没有其他等效的拉杆。