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Microelectrode puller requested for electrophysiologists at the university of waterloo

滑铁卢大学电生理学家需要微电极拉拔器

基本信息

批准号：
407023-2011
负责人：
Spafford, David
金额：
$ 1.57万
依托单位：
University of Waterloo
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2010
资助国家：
加拿大
起止时间：
2010-01-01 至 2011-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472608
关键词：
Microelectrode puller requested electrophysiologists university

项目摘要

The University of Waterloo Neuroscience Research Group including Dr. David Spafford and Dr. Matthijs van der Meer (Department of Biology) requests a Zeitz Model DMZ Universal Microelectrode Puller produced by Zeitz-Instruments Vertriebs GmbH in Germany. The Zeitz puller is available in North America through Dagan Corporation of Minneapolis, Minnesota for $21,560 CAD. The fabrication of microelectrodes for electrophysiological recording is an essential tool for neurophysiologists. A microelectrode puller is in use every day with research associates, post-doctoral fellows, graduate students and undergraduate students. There is only one micropipette puller at the University of Waterloo. It is a twelve year old, P-97 Flaming/Brown Micropipette Puller that has stopped functioning properly. It is imperative that a second puller be available because there is no other puller available for crafting microelectrodes at the University of Waterloo. We are looking to replace the old micropipette puller with a Zeitz Model DMZ Universal Microelectrode Puller. The Zeitz model is a new generation puller that will pull electrodes from glass and also automatically polish the tips for electrophyiological recording. There are vertical micropipette pullers which make less precise tips (Sutter P-30 / Kopf) and more expensive Laser-Based machines (Sutter P-2000), but neither of these suited to our needs. We include only one quote for the Zeitz Model puller because there is no other equivalent puller on the market.

滑铁卢大学神经科学研究小组，包括 David Spafford 博士和 Matthijs van der Meer 博士（生物系），请求使用德国 Zeitz-Instruments Vertriebs GmbH 生产的 Zeitz DMZ 型通用微电极拉拔器。 Zeitz 拉拔器在北美可通过明尼苏达州明尼阿波利斯市的 Dagan Corporation 购买，售价为 21,560 加元。用于电生理记录的微电极的制造是神经生理学家的重要工具。研究员、博士后、研究生和本科生每天都在使用微电极拉拔器。滑铁卢大学只有一名微量移液器拉拔器。这是一个已有 12 年历史的 P-97 火焰/棕色微量移液器拉拔器，已停止正常工作。必须有第二个拉拔器，因为滑铁卢大学没有其他拉拔器可用于制作微电极。我们希望用 Zeitz DMZ 型通用微电极拉拔器取代旧的微量移液器拉拔器。 Zeitz 型号是新一代拉拔器，可以从玻璃上拉出电极，并自动抛光电极尖以进行电生理记录。有垂直微量移液器拉拔器，其尖端精度较低（Sutter P-30 / Kopf）和更昂贵的激光机器（Sutter P-2000），但这些都不适合我们的需求。我们仅提供 Zeitz 型号拉拔器的一份报价，因为市场上没有其他同等的拉拔器。