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Microelectrode puller requested for electrophysiologists at the university of waterloo

滑铁卢大学电生理学家需要微电极拉拔器

基本信息

批准号：
407023-2011
负责人：
Spafford, David
金额：
$ 1.57万
依托单位：
University of Waterloo
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2010
资助国家：
加拿大
起止时间：
2010-01-01 至 2011-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472608
关键词：
Microelectrode puller requested electrophysiologists university

项目摘要

The University of Waterloo Neuroscience Research Group including Dr. David Spafford and Dr. Matthijs van der Meer (Department of Biology) requests a Zeitz Model DMZ Universal Microelectrode Puller produced by Zeitz-Instruments Vertriebs GmbH in Germany. The Zeitz puller is available in North America through Dagan Corporation of Minneapolis, Minnesota for $21,560 CAD. The fabrication of microelectrodes for electrophysiological recording is an essential tool for neurophysiologists. A microelectrode puller is in use every day with research associates, post-doctoral fellows, graduate students and undergraduate students. There is only one micropipette puller at the University of Waterloo. It is a twelve year old, P-97 Flaming/Brown Micropipette Puller that has stopped functioning properly. It is imperative that a second puller be available because there is no other puller available for crafting microelectrodes at the University of Waterloo. We are looking to replace the old micropipette puller with a Zeitz Model DMZ Universal Microelectrode Puller. The Zeitz model is a new generation puller that will pull electrodes from glass and also automatically polish the tips for electrophyiological recording. There are vertical micropipette pullers which make less precise tips (Sutter P-30 / Kopf) and more expensive Laser-Based machines (Sutter P-2000), but neither of these suited to our needs. We include only one quote for the Zeitz Model puller because there is no other equivalent puller on the market.

滑铁卢大学神经科学研究小组（包括大卫Spafford博士和Matthijs货车der梅尔博士（生物系））要求提供德国Zeitz-Instruments Vertriebs GmbH生产的泽茨型DMZ通用微电极拉拔器。该泽茨拉是可在北美通过达根公司明尼阿波利斯，明尼苏达州为21,560加元。用于电生理记录的微电极的制造是神经生理学家的重要工具。每天都有研究人员、博士后研究员、研究生和本科生使用微电极拉拔器。滑铁卢大学只有一台微量移液器。这是一个12岁的P-97火焰/棕色微量移液器拉已经停止正常工作。必须有第二个拉料器，因为滑铁卢大学没有其他拉料器可用于制作微电极。我们希望用泽茨型DMZ通用微电极拉拔器取代旧的微量移液器拉拔器。该泽茨模型是一个新一代的拉，将拉电极从玻璃，也自动抛光提示电泳记录。有垂直微量移液器拉取器，使不太精确的提示（萨特P-30 / Kopf）和更昂贵的激光为基础的机器（萨特P-2000），但这些都不适合我们的需要。我们只提供一个泽茨型拉料器的报价，因为市场上没有其他同等的拉料器。