Upgrade of U of Waterloo's primary confocal microscope with state-of-the-art correlative microscopy for co-applicants in 11 departments

为 11 个系的共同申请人升级滑铁卢大学的主共聚焦显微镜，配备最先进的相关显微镜

• 批准号: 473403-2015
• 负责人: Spafford, David
• 金额: $ 10.56万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564923
• 关键词: Upgrade; Waterloo; primary; confocal; microscope

We request purchase of Zeiss "Shuttle and Find" technology which serves as the interface for state-of-the-art correlative microscopy. Zeiss "Shuttle and Find" allows the taking of fluorescent confocal microscope image and then overlay the same ultrastructural image with a Zeiss Merlin electron microscope. The U of Waterloo is unique in Canada in possessing both Zeiss confocal and Zeiss scanning electron Zeiss microscopes (total cost >4 million dollars) that are compatible with state-of-the-art correlative microscopy. Besides the "Shuttle and Find" technology, we also require basic upgrades to Waterloo's primary confocal microsope which includes basic laser lines, bright-field transmitted light illumination and detection, and objectives.

我们要求购买蔡司的“Shuttle and Find”技术，作为最先进的相关显微镜的接口。蔡司“航天飞机和寻找”允许拍摄荧光共聚焦显微镜图像，然后用蔡司梅林电子显微镜覆盖相同的超微结构图像。滑铁卢大学在加拿大独一无二，拥有蔡司共焦显微镜和蔡司扫描电子蔡司显微镜(总成本&gt；400万美元)，与最先进的相关显微镜兼容。除了“航天飞机和发现”技术外，我们还需要对滑铁卢的主要共焦显微镜进行基本升级，包括基本的激光线路、明场透射光照明和检测以及物镜。