Upgrade of U of Waterloo's primary confocal microscope with state-of-the-art correlative microscopy for co-applicants in 11 departments

为 11 个系的共同申请人升级滑铁卢大学的主共聚焦显微镜，配备最先进的相关显微镜

• 批准号: 473403-2015
• 负责人: Spafford, David
• 金额: $ 10.56万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564923
• 关键词: Upgrade; Waterloo; primary; confocal; microscope

We request purchase of Zeiss "Shuttle and Find" technology which serves as the interface for state-of-the-art correlative microscopy. Zeiss "Shuttle and Find" allows the taking of fluorescent confocal microscope image and then overlay the same ultrastructural image with a Zeiss Merlin electron microscope. The U of Waterloo is unique in Canada in possessing both Zeiss confocal and Zeiss scanning electron Zeiss microscopes (total cost >4 million dollars) that are compatible with state-of-the-art correlative microscopy. Besides the "Shuttle and Find" technology, we also require basic upgrades to Waterloo's primary confocal microsope which includes basic laser lines, bright-field transmitted light illumination and detection, and objectives.

我们要求购买蔡司的“穿梭和查找”技术，作为国家的最先进的相关显微镜的接口。 Zeiss“Shuttle and Find”允许拍摄荧光共聚焦显微镜图像，然后用Zeiss Merlin电子显微镜覆盖相同的超微结构图像。 滑铁卢大学是加拿大唯一拥有蔡司共聚焦和蔡司扫描电子蔡司显微镜（总成本> 400万美元），与最先进的相关显微镜兼容。 除了“穿梭和发现”技术，我们还需要对滑铁卢的主要共焦显微镜进行基本升级，其中包括基本的激光线，亮场透射光照明和检测，以及物镜。