Isolation and characterization of plant insulators

植物绝缘体的分离和表征

• 批准号: 2869-2011
• 负责人: Johnson, Douglas
• 金额: $ 1.75万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=473640
• 关键词: Isolation; characterization; plant; insulators

Insulators are DNA elements that are able to block inappropriate or unwanted enhancer-promoter communication (enhancer blocker activity) and/or prevent the spread of repressive chromatin into active chromatin (barrier activity). Their action is mediated by protein(s) that recognize them leading to the recruitment of many proteins and ultimately the assembly "insulator bodies" the proposed "functional unit". Thus insulators represent an additional level of transcriptional control and their study represents an opportunity to understand a basic, universal mechanism of gene regulation.

绝缘体是能够阻止不适当或不想要的增强子-启动子通信(增强子阻断剂活性)和/或阻止抑制染色质扩散到活性染色质(屏障活性)的DNA元件。它们的作用是由识别它们的蛋白质(S)介导的，导致许多蛋白质的招募，并最终组装成“绝缘体”，即拟议的“功能单位”。因此，绝缘体代表了转录控制的额外水平，他们的研究提供了一个了解基本的、普遍的基因调控机制的机会。