Neuropeptides as regulators of sesquiterpenoid biosynthesis in arthropods

神经肽作为节肢动物倍半萜生物合成的调节剂

• 批准号: 9408-2009
• 负责人: Tobe, Stephen
• 金额: $ 10.56万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=474553
• 关键词: Neuropeptides; regulators; sesquiterpenoid; biosynthesis; arthropods

Juvenile hormone (JH) is the primary regulator of both the change in body form from larva to adult (metamorphosis) and the production of eggs (reproduction) in most insect species. JH must be present at appropriate times to stimulate the retention of larval characters or the growth of eggs. In its absence, these processes do not occur. Therefore the presence of JH at inappropriate times will disrupt the normal functioning of the insect. My laboratory has focused on the natural compounds that turn JH production on and off in pest species including cockroaches, moths and flies, with our major emphasis on the compounds responsible for turning JH production off. We have discovered a family of peptides (short chains of amino acids) termed allatostatins that are able to inhibit the production of JH. Some of our recent work has been directed to the development of mimics of these peptides that are resistant to breakdown and able to disrupt egg production. To this end, we have also been searching for additional allatostatin regulators of JH production in other pest species such as flies, since at present our knowledge of such compounds is largely confined to cockroaches. There are many related allatostatins in cockroaches but we know that these differ from those in other pest species such as moths and flies. This implies that allatostatins are highly specific, acting only on a narrow range of insects and it may be possible to exploit this specificity, by tailoring specific peptide mimics to act only on specific species. Our work has also focused on the receptors for allatostatins, the cellular keys responsible for the translation of the allatostatin signal since these molecules must be present in the insect's tissues to permit them to respond to the allatostatins. Expression of the receptors may permit the agrochemical industry to search for additional compounds that suppress JH production. We are also studying the JH-related compounds in the crustaceans, including lobster, shrimp and crab, with a view to identifying the regulators of crustacean reproduction and the peptides controlling their production. Such information may prove valuable to commercial aquaculture of lobster crab and shrimp, through the enhancement of growth and reproduction.

保幼激素（JH）是大多数昆虫从幼虫到成虫的身体形态变化（变态）和产卵（生殖）的主要调节因子。JH必须在适当的时间出现，以刺激幼虫特征的保留或卵的生长。如果没有它，这些过程就不会发生。因此，在不适当的时间出现JH会破坏昆虫的正常功能。我的实验室专注于在包括蟑螂，飞蛾和苍蝇在内的害虫物种中打开和关闭JH生产的天然化合物，我们主要强调负责关闭JH生产的化合物。我们已经发现了一个称为allatostatin的肽家族（氨基酸短链），能够抑制JH的产生。我们最近的一些工作已经针对这些肽的模拟物的开发，这些肽能够抵抗分解并能够破坏卵子生产。为此，我们也一直在寻找其他的allatostatin调节剂的JH生产在其他害虫物种，如苍蝇，因为目前我们的知识，这些化合物主要局限于蟑螂。蟑螂中有许多相关的allatostatin，但我们知道这些与其他害虫物种如飞蛾和苍蝇不同。这意味着allatostatin是高度特异性的，仅作用于窄范围的昆虫，并且可以通过定制特定的肽模拟物以仅作用于特定物种来利用这种特异性。我们的工作还集中在allatostatin的受体上，allatostatin是负责翻译allatostatin信号的细胞关键，因为这些分子必须存在于昆虫的组织中，以允许它们对allatostatin做出反应。受体的表达可能允许农业化学工业寻找抑制JH产生的其他化合物。我们还在研究甲壳类动物（包括龙虾、虾和蟹）中与JH有关的化合物，以期确定甲壳类动物繁殖的调节剂和控制其生产的肽。这些信息可能通过促进生长和繁殖，对龙虾、螃蟹和虾的商业化水产养殖很有价值。