Body condition, n-3/n-6 PUFA balance and the regulation of lipid and glucose metabolism in the mink

水貂体况、n-3/n-6 PUFA平衡及脂糖代谢调节

• 批准号: 203625-2008
• 负责人: RouvinenWatt, Kirsti
• 金额: $ 1.82万
• 依托单位: Nova Scotia Agricultural College
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=477210
• 关键词: Body; condition; PUFA; balance; regulation

Mink nursing sickness is a metabolic disorder characterized by impaired blood sugar regulation. It occurs when the demands for lactation require extensive mobilization of body energy reserves. Females that are either too heavy or too thin show higher variability in their blood sugar levels and have impaired health and reproduction. Rapid mobilization of body fat reserves is known to result in fatty liver (hepatic lipidosis), loss of the omega-3 polyunsaturated fatty acids (n-3 PUFA) and the development of insulin resistance. The long term goals of this research program are to understand the underlying biology of fatty liver syndrome and its association with mink nursing sickness. I plan to investigate the relationships that exist between body condition, fatty acid nutrition and management practices, such as feeding intensity and rapid weight loss, and the regulation of body fat and blood sugar metabolism in the mink. My objectives are to study the impact of the nutritional status on the metabolic response and the gene expression of key enzymes participating in body fat and blood sugar metabolism. Feeding experiments will be carried out where varying fatty acid profiles will be introduced to mink fed at two feeding intensity levels to induce lean and obese body conditions. The effects of rapid weight loss will be examined in response to short term fasting. I will also evaluate the level of oxidative stress and markers of inflammation in the mink with different n-3/n-6 PUFA profiles. In addition, I want to examine the formation of unfolded protein inclusions (Mallory bodies) in the liver tissue during fasting-induced hepatic lipidosis and more closely investigate their role in the fatty liver disease using cultured hepatocytes.

水貂哺乳病是一种以血糖调节受损为特征的代谢紊乱病。它发生在哺乳的需求需要广泛动员身体能量储备时。太重或太瘦的女性血糖水平变化较大，健康和生殖能力受损。已知身体脂肪储备的快速动员会导致脂肪肝（肝硬化）、ω-3多不饱和脂肪酸（n-3 PUFA）的损失和胰岛素抵抗的发展。这项研究计划的长期目标是了解脂肪肝综合征的潜在生物学及其与水貂护理病的关系。本研究旨在探讨水貂体况、脂肪酸营养与饲养强度、快速减重等管理措施之间的关系，以及水貂体脂肪和血糖代谢的调控。本研究的目的是研究营养状况对代谢反应的影响以及参与体脂和血糖代谢的关键酶的基因表达。饲养实验将进行不同的脂肪酸谱将被引入水貂饲养在两个饲养强度水平，以诱导精益和肥胖的身体状况。将在短期禁食的反应中检查快速体重减轻的影响。我还将评估具有不同n-3/n-6 PUFA谱的水貂的氧化应激水平和炎症标志物。此外，我想检查禁食诱导的肝脂质沉积期间肝组织中未折叠蛋白质内含物（马洛里体）的形成，并使用培养的肝细胞更仔细地研究它们在脂肪肝疾病中的作用。