Systematic mapping of a dosage suppression genetic

剂量抑制遗传的系统图谱

• 批准号: 204899-2011
• 负责人: Boone, Charles
• 金额: $ 2.91万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=477400
• 关键词: Systematic; mapping; dosage; suppression; genetic

Increasing gene dosage provides a powerful means of probing cellular functions, such as growth or cell division. The sophisticated molecular genetic methods available with yeast, makes this organism an ideal model system for studying fundamental cellular functions. In total, yeast cells have ~6000 different genes and a subset of ~1000 of these genes are each essential because their deletion kills the cell. We developed a novel system to identify yeast genes whose increased activity can override and suppress the lethality associated with the loss-of-function of a given essential gene. Comprehensive mapping of these genetic interactions will generate a functional wiring diagram of the cell because it defines genes that work together to control essential cellular functions. Because many cellular functions are conserved from yeast to human, the resultant genetic network will be informative about genetics underlying human diseases and it will be useful for the development of new therapeutics.

增加基因剂量提供了探测细胞功能（如生长或细胞分裂）的有力手段。酵母的复杂分子遗传学方法使这种生物体成为研究基本细胞功能的理想模型系统。 总的来说，酵母细胞有大约6000个不同的基因，其中大约1000个基因的子集是必不可少的，因为它们的缺失会杀死细胞。 我们开发了一种新的系统来识别酵母基因，其增加的活性可以覆盖和抑制与给定必需基因的功能丧失相关的致死性。这些基因相互作用的全面映射将生成细胞的功能布线图，因为它定义了共同控制基本细胞功能的基因。 由于许多细胞功能从酵母到人类都是保守的，因此所产生的遗传网络将提供有关人类疾病遗传学的信息，并将有助于开发新的治疗方法。