The checkpoint role of budding yeast dbf4/cdc7

芽殖酵母dbf4/cdc7的检查点作用

• 批准号: 238392-2010
• 负责人: Duncker, Bernard
• 金额: $ 1.97万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=478836
• 关键词: checkpoint; role; budding; yeast; dbf4

My research group is using budding yeast in studies of cell division. We are currently focusing our investigations on identifying and characterizing protein factors that control the initiation of DNA replication (duplication of the genetic material). Budding yeast has proven to be a very useful organism for such studies because it is one of the few organisms for which the starting points for DNA replication (origins of replication) have been well characterized. This has facilitated the identification of numerous protein factors that associate with replication origins. Dbf4 and Cdc7 are two such proteins which work together to trigger replication. In addition to their function in DNA replication, Dbf4 and Cdc7 are important for cellular 'checkpoint' functions, which prevent duplication of DNA when it has been damaged. Research in my lab is centered on a better understanding of the way these proteins work together to carry out their essential roles in cell division. The results of these studies will help us better determine how organisms, including humans, grow and develop, as well us help us understand the consequences of exposure to environmental pollutants that cause DNA damage.

我的研究小组正在用芽殖酵母研究细胞分裂。我们目前的研究重点是识别和表征控制DNA复制（遗传物质复制）启动的蛋白质因子。芽殖酵母已被证明是一种非常有用的生物体，因为它是为数不多的生物体之一，其DNA复制的起点（复制起点）已得到很好的表征。这促进了与复制起点相关的许多蛋白质因子的鉴定。Dbf4和Cdc7是两种这样的蛋白质，它们一起工作以触发复制。除了它们在DNA复制中的功能外，Dbf4和Cdc7对于细胞的“检查点”功能也很重要，当DNA被损坏时，它们可以防止DNA复制。我实验室的研究集中在更好地理解这些蛋白质如何协同工作，以发挥它们在细胞分裂中的重要作用。这些研究的结果将帮助我们更好地确定包括人类在内的生物体如何生长和发育，并帮助我们了解暴露于环境污染物导致DNA损伤的后果。