The development of a two-colour flow cytometric assay for the detection of whole cell biosensors in environmental samples.

开发双色流式细胞术检测环境样品中的全细胞生物传感器。

• 批准号: ARC : LX0347172
• 负责人: Prof Duncan Veal
• 金额: $ 0.96万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage - International
• 财政年份: 2002
• 资助国家: 澳大利亚
• 起止时间: 2002-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/the-development-two-environmental-samples/72286
• 关键词: development; two; colour; flow; cytometric

The development of a two-colour flow cytometric assay for the detection of whole cell biosensors in environmental samples. Macquarie University and the University of Copenhagen have expertise in fluorescence detection and whole cell biosensors respectively. The project will take advantage of these skills and develop a sensitive assay for monitoring biosensor bacteria in soil. The technology will be significant as it will enable real time analysis of antibiotic production in situ through the detection of GFP expression. This work will then be used to isolate new antibiotic produces and will be extended to research into the bioavailability of toxic compounds and stress. An existing collaboration between the two institutions will be extended enabling the transfer and application of biosensor technology to Australia.

开发双色流式细胞术检测环境样品中的全细胞生物传感器。麦考瑞大学和哥本哈根大学分别在荧光检测和全细胞生物传感器方面拥有专业知识。该项目将利用这些技能，开发一种灵敏的检测方法来监测土壤中的生物传感器细菌。该技术意义重大，因为它将通过检测 GFP 表达来实时分析抗生素的原位生产。这项工作随后将用于分离新的抗生素产品，并将扩展到有毒化合物和压力的生物利用度的研究。两个机构之间现有的合作将得到扩展，从而实现生物传感器技术向澳大利亚的转让和应用。