Coupling metabolomic and proteomic approaches in profiling endobiotics metabolism

代谢组学和蛋白质组学方法相结合分析内生素代谢

• 批准号: 402213-2011
• 负责人: Barbier, Olivier
• 金额: $ 2.19万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Group
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=487976
• 关键词: Coupling; metabolomic; proteomic; approaches; profiling

Numerous endogenous substances exert major biological roles. The maintenance of these roles is ensured by adequate levels of synthesis and metabolism. However, studying their metabolism is currently limited by the absence of methods for the measurement of the enzymes involved in these metabolic processes. However, novel technologies, such as proteomic and metabolomic now allow the specific identification of proteins and endogenous substance metabolites, respectively. The purpose of our novel research program is to pair these new technologies in order to generate a serie of quantification tools that will be useful in investigating how genetic and environmental factors can alter the metabolism and function of biologically active endogenous substances. Indeed, these tools will provide for the first time the possibility of deciphering the exact contribution of EME isoforms in cellular response to genetic or environmental challenges.

许多内源性物质发挥主要的生物作用。这些作用的维持是由足够水平的合成和代谢保证的。然而，研究它们的代谢目前受到缺乏测量参与这些代谢过程的酶的方法的限制。然而，新的技术，如蛋白质组学和代谢组学，现在允许蛋白质和内源性物质代谢物的具体鉴定，分别。我们新研究计划的目的是将这些新技术配对，以产生一系列量化工具，这些工具将有助于研究遗传和环境因素如何改变生物活性内源性物质的代谢和功能。事实上，这些工具将首次提供破译EME亚型在细胞对遗传或环境挑战的反应中的确切贡献的可能性。