COUPLING METABOLOMIC AND PROTEOMIC APPROACHES IN PROFILING ENDOBIOTICS METABOLISM

代谢组学和蛋白质组学方法联用分析内生菌代谢

• 批准号: 402213-2012
• 负责人: Barbier, Olivier
• 金额: $ 2.84万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Group
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=573197
• 关键词: COUPLING; METABOLOMIC; PROTEOMIC; APPROACHES; PROFILING

Endobiotics are small molecules formed in the body that exert essential biological roles in development and adult physiology. The maintenance of such roles is ensured by adequate levels of synthesis and metabolism. However, the exact contribution of metabolism in the control of endobiotics activities is still unclear, since the researchers working in this field face a major limitation: i.e. the absence of accurate methods for the selective and sensitive measurement of metabolic enzymes. In the last 5 years, novel state of-the-art technologies, such as LC-MS/MS, have favoured the emergence of novel scientific disciplines named proteomic and metabolomic. A significant part of these disciplines is dedicated to the specific identification of proteins and endobiotics. The purpose of this novel research program is to associate these new disciplines in order to generate a series of quantification tools (called preotomic/metabolomic pairs) that will be useful in investigating how genetic and environmental factors can alter the metabolism and function of biologically active endogenous substances. Indeed, these tools will provide for the first time the possibility of deciphering the exact contribution of each metabolic enzyme in the cellular response to genetic or environmental challenges.

内生菌是在体内形成的小分子，在发育和成人生理学中发挥重要的生物学作用。这种作用的维持是通过适当水平的合成和代谢来保证的。然而，代谢在控制内生菌活性中的确切作用仍然不清楚，因为在该领域工作的研究人员面临着一个主要的限制：即缺乏用于代谢酶的选择性和灵敏度测量的准确方法。在过去的5年中，最先进的新技术，如LC-MS/MS，有利于蛋白质组学和代谢组学等新学科的出现。这些学科的一个重要部分是致力于蛋白质和内生菌的具体鉴定。这项新的研究计划的目的是将这些新学科联系起来，以产生一系列量化工具（称为preotomic/metabolomic pairs），这些工具将有助于研究遗传和环境因素如何改变生物活性内源性物质的代谢和功能。事实上，这些工具将首次提供破译每种代谢酶在细胞对遗传或环境挑战的反应中的确切贡献的可能性。