Structure-function study of the CFTR (ABCC7) chloride channel

CFTR (ABCC7) 氯离子通道的结构-功能研究

• 批准号: 402229-2011
• 负责人: Chappe, Valerie
• 金额: $ 2.19万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=487984
• 关键词: Structure; function; study; CFTR; ABCC7

ABC transporters are a family of protein which play numerous important roles in organism physiology, from bacteria to human. They translocate a wide variety of molecules such as sugars, vitamins and even small proteins in different compartment of the cell or outside of cells. The 48 human ABC proteins are mostly exporters involved in secretions , drug detoxification, and the immune system. Structural studies have been instrumental in discovering the 3D structure of bacterial ABC proteins, providing numerous information on how they work. Unfortunately, for more complex ABC proteins, like the human ones, which are involved in many important physiological functions, it is not possible to purify them and obtain a precise 3D structure by crystallisation. Most of their characteristics and functional properties are thus determined by comparison with bacterial ABC transporters. As a result, current models need to be further tested and evaluated by functional and physiologically relevant studies, such as those we propose in our research program.

ABC转运蛋白是一个蛋白家族，在从细菌到人类的生物生理中起着许多重要的作用。它们将各种各样的分子，如糖、维生素，甚至小蛋白质，转移到细胞内或细胞外的不同区域。48种人类ABC蛋白主要是参与分泌物、药物解毒和免疫系统的出口蛋白。结构研究有助于发现细菌ABC蛋白的三维结构，提供了关于它们如何工作的大量信息。不幸的是，对于更复杂的ABC蛋白，比如参与许多重要生理功能的人类ABC蛋白，不可能提纯它们并通过结晶获得精确的3D结构。因此，它们的大多数特性和功能特性是通过与细菌ABC转运体的比较来确定的。因此，目前的模型需要通过功能和生理学相关的研究来进一步测试和评估，例如我们在研究计划中提出的研究。