Structure/function study of the CFTR (ABCC7) chloride channel

CFTR (ABCC7) 氯离子通道的结构/功能研究

• 批准号: 402229-2012
• 负责人: Chappe, Valerie
• 金额: $ 1.89万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=529482
• 关键词: Structure; function; study; CFTR; ABCC7

ABC transporters are a family of proteins which play numerous important roles in organisms' physiology, from bacteria to humans. They transport a wide variety of molecules such as sugars, vitamins and even small proteins from different compartments of the cell or outside of cells. The 48 human ABC proteins are mostly involved in secretions, drug detoxification, and identification of pathogens by the immune system. Structural studies of bacterial ABC proteins have been instrumental in discovering their 3D structure and providing numerous information on how they work. Unfortunately, for complex ABC proteins, such as the ones fund in humans, current advanced technologies are not developed enoughto permit extraction from cells in a way which would enable researchers to visualise their precise 3D structure. Most of the characteristics and functional properties of human ABC proteins are deduced from models based on predicted similarities with bacterial ABCs. Current models of human ABC proteins thus need to be validated by further testing for their functional and physiological relevance. This is the type of research that we do in our lab.

ABC转运蛋白是一个蛋白质家族，在从细菌到人类的生物生理学中发挥着许多重要作用。它们从细胞的不同隔室或细胞外运输各种分子，如糖，维生素甚至小蛋白质。48种人类ABC蛋白主要参与分泌物、药物解毒和免疫系统对病原体的识别。细菌ABC蛋白的结构研究有助于发现其3D结构，并提供有关其工作方式的许多信息。不幸的是，对于复杂的ABC蛋白质，例如在人类中发现的蛋白质，目前的先进技术还不足以允许从细胞中提取，从而使研究人员能够可视化其精确的3D结构。人类ABC蛋白的大多数特征和功能特性是根据与细菌ABC的预测相似性从模型中推导出来的。因此，需要通过进一步测试其功能和生理相关性来验证人类ABC蛋白的当前模型。这就是我们在实验室里做的研究。