Large volume preparative ultracentrifuge for the enhancement of membrane biophysics research at Guelph

大容量制备型超速离心机用于加强圭尔夫膜生物物理学研究

• 批准号: 422205-2012
• 负责人: Brown, Leonid
• 金额: $ 9.52万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495746
• 关键词: Large; volume; preparative; ultracentrifuge; enhancement

Biological membranes are multifunctional proteolipid complexes, responsible for the multitude of vital cellular functions, including sensing, bioenergetics, transport, and enzymatic activities. Membrane biophysics group at Guelph studes structure and dynamics of lipid membranes, structure/function relationship in membrane transporters and photosensors, as well as interaction of proteins and peptides with membranes. Preparation of the samples for biophysical studies by advanced spectroscopic and microscopic techniques includes ultracentrifugation of relatively large volumes, this is why having a dedicated large volume ultracentrifuge is essential. The requested ultracentrifuge will advance research of many graduate and undergraduate students, as well as postdoctoral fellows, who work on fundamental projects related, among other topics, to effects of cholesterol on biological membranes, medically important human membrane proteins, bacterial pathogenesis, and mechanism of action of antimicrobial compounds.

生物膜是多功能的蛋白脂质复合物，负责许多重要的细胞功能，包括传感，生物能量学，运输和酶活性。圭尔夫的膜生物物理学小组研究脂质膜的结构和动力学，膜转运蛋白和光传感器的结构/功能关系，以及蛋白质和肽与膜的相互作用。通过先进的光谱和显微技术制备用于生物物理研究的样品包括相对大体积的超离心，这就是为什么具有专用的大体积超离心是必不可少的。所要求的超速离心机将推进许多研究生和本科生以及博士后研究员的研究，他们从事的基础项目涉及胆固醇对生物膜的影响、医学上重要的人类膜蛋白、细菌发病机制和抗微生物化合物的作用机制等主题。