Memory encoding and storage in cortical circuits: electrophysiological studies

皮层回路中的记忆编码和存储：电生理学研究

• 批准号: 227273-2009
• 负责人: Chapman, Andrew
• 金额: $ 2.91万
• 依托单位: Concordia University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=497266
• 关键词: Memory; encoding; storage; cortical; circuits

A new memory requires the formation of a new memory trace, or "engram", in the brain. Memory traces are thought to consist of networks of simultaneously activated neurons, and a major mechanism through which new networks can be formed is through long-lasting increases (or decreases) in the strength of synaptic connections between neurons. My research is aimed at understanding how new memory traces are formed through long-lasting changes in the strength of synaptic connections between brain cells. The parahippocampal cortex and neocortex are regions of the brain that are crucial for normal memory formation. The parahippocampal area is thought to combine different types of sensory input during memory encoding. Memory is then thought to be stored more permanently through connections with other parts of the brain including the neocortex. To better understand the fundamental mechanisms that mediate memory formation, I use various electrophysiological and pharmacological techniques to determine how synaptic strengths can be altered in the parahippocampal and neocortical regions of the rat brain. There are two primary lines of research. The first line of research is aimed at understanding how the entorhinal cortex (an important parahippocampal area) processes and encodes sensory input. Experiments examine how temporal patterns of activity increase or decrease synaptic strength, and how activity in the entorhinal cortex can be modulated by the parasubiculum and perirhinal cortex. These experiments are important because they can indicate how sensory information is processed by neural circuits during the encoding of a new memory. The second line of research is aimed at understanding changes that occur in the neocortex during memory storage. Experiments will focus on identifying elements of the neocortical circuit that are affected by a cellular model of learning, and characterizing the cortical EEG rhythms and morphological changes in neurons that can lead to long-lasting changes in synaptic strength. This line of research may help us understand how memories can be stored in the neocortex for long periods of time.

一个新的记忆需要在大脑中形成一个新的记忆痕迹，或“印记”。记忆痕迹被认为是由同时激活的神经元网络组成的，而形成新网络的一个主要机制是通过神经元之间突触连接强度的长期增加（或减少）。我的研究旨在了解新的记忆痕迹是如何通过脑细胞之间突触连接强度的长期变化形成的。海马旁皮质和新皮质是大脑中对正常记忆形成至关重要的区域。海马旁区被认为在记忆编码过程中结合联合收割机不同类型的感觉输入。记忆被认为是通过与大脑其他部分（包括新皮层）的连接而更永久地存储的。为了更好地了解介导记忆形成的基本机制，我使用各种电生理学和药理学技术来确定突触强度如何在大鼠大脑的海马旁区和新皮质区改变。有两条主要的研究路线。研究的第一条线旨在了解内嗅皮层（一个重要的海马旁区）如何处理和编码感觉输入。实验研究了活动的时间模式如何增加或减少突触强度，以及内嗅皮层的活动如何被旁视丘和嗅周皮层调制。这些实验很重要，因为它们可以表明在编码新记忆的过程中，神经回路是如何处理感官信息的。第二条研究路线旨在了解记忆储存期间新皮层发生的变化。实验将侧重于识别受学习的细胞模型影响的新皮层电路的元素，并表征皮层EEG节律和神经元的形态变化，这些变化可能导致突触强度的长期变化。这一系列的研究可能会帮助我们理解记忆是如何长时间储存在大脑皮层中的。