Role of the adaptor protein CASP

接头蛋白 CASP 的作用

• 批准号: 36594-2009
• 负责人: Pohajdak, Bill
• 金额: $ 2.33万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498070
• 关键词: Role; adaptor; protein; CASP

Movement of proteins and organelles within a cell is a well-regulated process involving many molecular interactions. We discovered several proteins that are involved in this movement process. We recently found a novel interaction between two proteins in lymphocytes. These proteins are involved in moving vesicles to the area of the lymphocyte that is in contact with tumour cells. We will attempt to isolate other proteins that bind these proteins and determine how they are involved in movement of vesicles in lymphocytes. One protein is also cleaved when the cells potentially commit suicide. We would like to determine if this mechanism is used to kill and remove infected lymphocytes. Another structurally related protein to our protein was also found in nerve cells. Recently we have found it to be also expressed in lymphocytes. This protein may have different lengths. We will determine if these similar proteins can substitute for each other. The roles of these proteins in moving vesicles to the immune synapse and also their role in cell motility will be explored. Our long term goals are to determine how these proteins participate in coordinating vesicular traffic based upon our novel model.

蛋白质和细胞器在细胞内的运动是一个涉及许多分子相互作用的受良好调控的过程。我们发现了几种参与这一运动过程的蛋白质。我们最近在淋巴细胞中发现了两种蛋白质之间的新相互作用。这些蛋白质参与将囊泡移动到与肿瘤细胞接触的淋巴细胞区域。我们将尝试分离出与这些蛋白质结合的其他蛋白质，并确定它们如何参与淋巴细胞中小泡的运动。当细胞可能自杀时，一种蛋白质也被切割。我们想确定这种机制是否用于杀死和清除受感染的淋巴细胞。 在神经细胞中也发现了另一种与我们的蛋白质结构相关的蛋白质。最近我们发现它也在淋巴细胞中表达。这种蛋白质可能具有不同的长度。 我们将确定这些相似的蛋白质是否可以相互替代。这些蛋白质的作用，在移动囊泡的免疫突触，也将探讨其在细胞运动的作用。我们的长期目标是根据我们的新模型确定这些蛋白质如何参与协调囊泡交通。