Functional characterization of noncollagenous bone matrix proteins

非胶原骨基质蛋白的功能表征

• 批准号: 355793-2009
• 负责人: Nanci, Antonio
• 金额: $ 2.33万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498098
• 关键词: Functional; characterization; noncollagenous; bone; matrix

In calcified tissues, noncollagenous proteins are dispersed throughout the extracellular matrix and although they represent minor constituents, they exert major regulatory influences on the deposition of mineral and the activity of the cells responsible for the formation of the tissue. Elucidating the molecular mechanisms by which implicated proteins express their function is the next challenge scientists face in understanding how the body functions and how disease alters this pattern. Despite the relative abundance of biochemical data on noncollagenous proteins, their precise role in vivo, where the whole animal biology comes into play, remains to be determined. Information on the activities and properties of these proteins has been largely deduced from their temporo-spatial pattern of expression and often tested experimentally using in vitro systems in which, at most, few parameters can be evaluated at a time. Gene transfer will be used to create local transgenic conditions in a rat model of intramembranous bone formation and examine the role of specific functional domains of major noncollagenous bone matrix proteins. This approach offers a simple system in which portion and domains of a molecule can be tested for function and therapeutic potential in an environment that reflects the complex nature of the body. Such an approach is less time consuming and costly then creating a knockout

在钙化组织中，非胶原蛋白分散在整个细胞外基质中，尽管它们只是次要成分，但它们对矿物质的沉积和负责组织形成的细胞的活性具有重要的调节作用。阐明相关蛋白表达其功能的分子机制是科学家们在了解身体如何运作以及疾病如何改变这种模式方面面临的下一个挑战。尽管关于非胶原蛋白的生物化学数据相对丰富，但它们在体内的确切作用，即整个动物生物学发挥作用的地方，仍有待确定。关于这些蛋白质的活性和特性的信息在很大程度上是从它们的时空表达模式推断出来的，并且经常使用体外系统进行实验测试，其中一次最多只能评估很少的参数。基因转移将用于在大鼠膜内骨形成模型中创建局部转基因条件，并检查主要非胶原骨基质蛋白的特定功能域的作用。这种方法提供了一种简单的系统，可以在反映身体复杂本质的环境中测试分子的部分和结构域的功能和治疗潜力。这种方法比创造一个击倒对手更节省时间和成本