Functional genomics methodology for the study of environmental sensing in streptococci

用于研究链球菌环境传感的功能基因组学方法

• 批准号: 262153-2008
• 负责人: McCormick, John
• 金额: $ 2.04万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498168
• 关键词: Functional; genomics; methodology; study; environmental

Understanding mechanisms by how microbes are able to sense and interact with their environment remains a major challenge, and as such, a comprehensive and detailed understanding as to how most bacteria respond to environmental signals remains incomplete. This proposal uses genetic approaches to determine what streptococcal genes are turned on in specific environments. The methodology uses "genetic trickery", where upon gene induction the bacteria are "tagged", and we can use this information to predict genes that are required for bacterial survival within a specific niche. The information will then be utilized to design biochemical methods to determine the identity of the signals being sensed by the streptococci.

了解微生物如何感知环境并与环境相互作用的机制仍然是一个重大挑战，因此，对大多数细菌如何响应环境信号的全面而详细的了解仍然不完整。该提案使用遗传学方法来确定在特定环境中哪些链球菌基因被打开。该方法使用“遗传诡计”，在基因诱导后，细菌被“标记”，我们可以使用这些信息来预测细菌在特定生态位内生存所需的基因。这些信息将被用来设计生物化学方法，以确定链球菌感受到的信号的身份。