High Resolution Imaging Suite for Analysis of Development

绘制发育过程和基因表达的能力需要高分辨率显微镜在子宫内，以及在器官和细胞培养。 成像的最新进展还具有除了静态成像之外还能够提供功能数据的优点。 这项建议的目的是建立一个显微镜套件，可用于映射发育过程和基因表达，通过几种不同的方式。 拟议的设备将为我们提供一个能力，以确定随着时间的推移，在发展青蛙和小鼠模型的基因表达。宏观荧光显微镜将通过允许全胚胎分析以及评估单个细胞表达的能力来分析转基因动物中的荧光基因表达。 高端单色数码相机与高功率物镜和多个荧光染料滤光片相结合，提供了同时评估多个荧光团的亚细胞定位的能力。 最后，我们需要一个异氟烷室麻醉小鼠，从而允许在活体动物的组织发育的功能分析，通过高分辨率超声。 通过将这种基础设施与当前的显微镜核心相结合，我们将能够以我们以前无法做到的方式检查发育过程。

The ability to map developmental processes and gene expression requires high resolution microscopy both in utero, as well as in organ and cell cultures. Recent advances in imaging also have an advantage in being able to provide functional data in addition to static imaging. The purpose of this proposal is to establish a microscopy suite that can be used to map developmental processes and gene expression through several different modalities. The proposed equipment will provide us with an ability to identify the expression of genes over time in developing frog and mouse models. A macrofluorescent microscope will enable analysis of fluorescent gene expression in transgenic animals by allowing whole embryo analysis, as well as the ability to assess individual cell expression. A high end monochrome digital camera combined with a high power objective and multiple fluorochrome filters provides the ability to assess subcellular localization of multiple fluorophores at the same time. Finally, we require an isofluorane chamber for anesthetizing mice, thereby allowing for functional analysis of tissue development in live animals by high resolution ultrasound. By combining this infrastructure with the current microscopy core, we will be able to examine developmental processes in a fashion that we have previously be unable to do.