Role of annexin A2 as a scavenger of reactive oxygen species

膜联蛋白 A2 作为活性氧清除剂的作用

• 批准号: 402415-2012
• 负责人: Waisman, David
• 金额: $ 1.89万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=505742
• 关键词: Role; annexin; A2; scavenger; reactive

Reactive Oxygen Species (ROS) are molecules produced during metabolism that damage a number of cellular components, including the DNA, proteins and lipids. The DNA contains all the genetic information for the transmission of inherited traits from an existing cell to a new cell, while proteins are required for the structure, function and regulation of the body's cells, tissues and organs and lipids are not only a source of fuel but also an important constituent of the structure of cells. Harm to any of these components can lead to cell death. To protect cells from the deleterious effects of ROS, cells release antioxidant proteins and substances. Preliminary work from our laboratory has shown that depletion of the protein annexin A2 (also known as ANXA2) leads to an increase in ROS levels in stressed cells. Since ANXA2 is a very abundant and widely distributed cellular protein, we hypothesized that ANXA2 might play a major role in protecting cells from oxidative stress by absorbing ROS like a sponge absorbs water. With this project we will investigate in detail the mechanisms by which ANXA2 protects the cells from these harmful ROS molecules.

活性氧种(ROS)是新陈代谢过程中产生的分子，它会破坏许多细胞成分，包括DNA、蛋白质和脂质。DNA包含了将遗传特征从现有细胞传递到新细胞的所有遗传信息，而蛋白质是人体细胞、组织和器官的结构、功能和调节所必需的，而脂质不仅是燃料的来源，也是细胞结构的重要组成部分。这些成分中的任何一种受到伤害都可能导致细胞死亡。为了保护细胞免受ROS的有害影响，细胞释放抗氧化蛋白质和物质。我们实验室的初步工作表明，蛋白质Annexin A2(也称为ANXA2)的耗尽会导致应激细胞中ROS水平的增加。由于ANXA2是一种非常丰富和广泛分布的细胞蛋白，我们推测ANXA2可能通过像海绵吸收水分一样吸收ROS来保护细胞免受氧化应激的影响。在这个项目中，我们将详细研究ANXA2保护细胞免受这些有害ROS分子伤害的机制。