Functional synergy between the DNA topology and DNA repair machineries during the initiation of DNA replication

许多正常和疾病相关过程的一个基本组成部分是DNA的复制，DNA是所有活细胞的遗传物质。尽管它的重要性和多年来发现的许多分子参与其中，DNA复制的调控仍然是未知的。在以前的研究中，Zannis-Hadjopoulos博士的团队确定了Ku，一种参与DNA修复的多功能蛋白质，也通过与其他蛋白质的相互作用参与DNA复制的调节，其中包括拓扑异构酶II，一种参与维持DNA正确结构的蛋白质。他们现在建议分析Ku与拓扑异构酶II相互作用的方式，并研究这种蛋白质复合物参与DNA复制的机制。

A fundamental component of many normal and disease-related processes is the replication of DNA, the genetic material of all living cells. Despite its importance and the discovery of many molecules that are involved in it over the years, the regulation of DNA replication is still largely unknown. In previous studies, Dr. Zannis-Hadjopoulos' team identified Ku, a multifunctional protein involved in DNA repair, as involved also in the regulation of DNA replication through its interaction with other proteins, among them topoisomerase II, a protein that is involved in maintaining the correct architecture of the DNA. They now propose to analyze the manner by which Ku interacts with topoisomerase II and examine the mechanism by which this protein complex is involved in DNA replication.