Making sense of antisense: the activities of RNA-dependent RNA polymerases in endogenous RNAi

理解反义：内源性 RNAi 中 RNA 依赖性 RNA 聚合酶的活性

• 批准号: 341457-2012
• 负责人: Duchaine, Thomas
• 金额: $ 2.48万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=506051
• 关键词: Making; sense; antisense; activities; RNA

In RNA-mediated interference (RNAi), small non-coding RNAs (siRNAs) are generated to direct specific gene silencing as they program a variety of effector complexes to degrade cognate messenger RNAs, or silence their transcription. Many RNAi-like pathways function endogenously in plants, fungi, yeast, and animals. Endogenous (endo)RNAi refers to mechanisms wherein siRNAs are derived from and converge back on endogenous loci. While the gene silencing steps of endoRNAi are becoming clear, little is known about their initiation, and on the basis of their specificity. RNA-dependent RNA polymerases (RdRP) are often central to initiation of endoRNAi. With this grant, we will examine the RRF-3 RdRP to decipher the basis for its specificity, and elucidate the mechanism of ERI endoRNAi initiation.

在RNA介导的干扰（RNAi）中，产生小的非编码RNA（siRNA）以指导特定的基因沉默，因为它们编程多种效应复合物以降解同源信使RNA或沉默其转录。许多RNAi样途径在植物、真菌、酵母和动物中内源性发挥作用。内源性（endo）RNAi是指其中siRNA来源于内源基因座并会聚回内源基因座的机制。虽然endoRNAi的基因沉默步骤变得越来越清楚，但对它们的起始以及基于它们的特异性知之甚少。RNA依赖性RNA聚合酶（RdRP）通常是endoRNAi起始的核心。有了这笔资金，我们将研究RRF-3 RdRP以破译其特异性的基础，并阐明ERI endoRNAi启动的机制。