Regulation of mitochondrial metabolism by the phosphorylation system and the complex IV and its impact on oxidative stress

磷酸化系统和复合体IV对线粒体代谢的调节及其对氧化应激的影响

• 批准号: 402636-2012
• 负责人: Lemieux, Hélène
• 金额: $ 2.11万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=506542
• 关键词: Regulation; mitochondrial; metabolism; phosphorylation; system

A large fraction of energy needed for heart and skeletal muscle contraction comes from oxidative phosphorylation (OXPHOS), a process that takes place in the inner mitochondrial membrane. OXPHOS couple the transfer of electrons in the electron transport system (ETS) to the movement of protons in the intermembrane space. The proton gradient formed is accumulated between the two mitochondrial membranes and is utilized by the phosphorylation system to produce energy in the form of ATP. The energy produced depends not only from the efficiency of electron transport between the complexes I to IV of the ETS but also of the ability of the phosphorylation system to convert the proton gradient into energy. For instance, previous studies showed that the phosphorylation system exert a strong control on OXPHOS in the human heart and skeletal muscle, whereas this particular characteristic is not shared with commonly used animal models such as mice and rats.

心脏和骨骼肌收缩所需的很大一部分能量来自氧化磷酸化(OXPHOS)，这是一个发生在线粒体内膜的过程。OXPHOS将电子传输系统(ETS)中的电子转移与质子在膜间隙中的运动相结合。形成的质子梯度在两个线粒体膜之间积累，并被磷酸化系统利用以ATP的形式产生能量。产生的能量不仅取决于ETS的络合物I到IV之间的电子传递效率，而且还取决于磷酸化体系将质子梯度转换为能量的能力。例如，以前的研究表明，磷酸化系统对人类心脏和骨骼肌中的OXPHOS起着强有力的控制作用，而这一特殊特征在常用的动物模型如小鼠和大鼠中并不相同。