Nutrient cycling in lakes

湖泊的养分循环

• 批准号: 8110-2011
• 负责人: Taylor, William
• 金额: $ 2.77万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=507040
• 关键词: Nutrient; cycling; lakes

My research program concerns microbial food webs and nutrient cycles, and in this grant cycle my lab will target four areas. The first area concerns dissolved organic phosphorus (DOP) in lake water. This is the largest dissolved fraction of this limiting nutrient, but our recent work demonstrates that it is mostly derived during the filtration step of sample preparation, probably by cell breakage. Our hypothesis is that truly dissolved organic P is also derived from cell breakage, but due to natural mechanisms such as viral lysis and grazing. To complete this line of research we will test the hypotheses that the high molecular weight fraction of DOP is mostly nucleic acids, and that these become PO4 via low molecular weight DOP that is mostly nucleotides and other substrates available to plankton via cell-membrane associated phosphatases.

我的研究项目涉及微生物食物网和营养循环，在这个资助周期中，我的实验室将瞄准四个领域。 第一个领域涉及湖水中溶解有机磷（DOP）。 这是这种限制性营养素的最大溶解部分，但我们最近的工作表明，它主要是在样品制备的过滤步骤中获得的，可能是通过细胞破碎。 我们的假设是，真正溶解的有机磷也来自细胞破碎，但由于自然机制，如病毒裂解和放牧。 为了完成这一系列的研究，我们将测试的假设，DOP的高分子量部分主要是核酸，这些成为PO4通过低分子量DOP，主要是核苷酸和其他底物可用于浮游生物通过细胞膜相关的磷酸酶。