Purchase of a Steam Sterilizer (Autoclave)

购买蒸汽灭菌器（高压灭菌器）

• 批准号: 439753-2013
• 负责人: Daugulis, Andrew
• 金额: $ 3.2万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=509713
• 关键词: Purchase; Steam; Sterilizer; Autoclave

We are requesting funds for a steam sterilizer (or autoclave) in support of the research of Drs Andrew Daugulis (AD) and Kimberly Woodhouse (KW), both of whom work with biological systems, requiring sterile conditions that are provided by this equipment. The sterilizer is intended to replace one that is now more than 10 years old, and which requires regular and expensive maintenance. AD cultivates microorganisms for the production of high value neutraceuticals, as well as for the bioremediation of toxic environmental contaminants. In all cases, to ensure that there is no contamination with infecting microorganisms, all materials (glassware, culture vessels, growth medium) must be sterilized prior to use, which is the purpose of the autoclave. KW undertakes research that involves the production of materials that mimic nature in tissue engineering and regenerative medicine applications. These molecules are pieces of the human protein elastin that is found in arteries and other tissues that provides elasticity. KW uses portions of a human elastin gene which is inserted into the DNA of bacteria, which are then grown in culture vessels. This work requires absolute sterility of materials, again provided by use of the autoclave. Moreover, there cannot be any cross-contamination between wild strains of microorganisms (AD) and genetically modified strains of E coli cells (KW) between the 2 Research Groups in the shared research facilities, which would be prevented through the employment of an autoclave. Finally, it is a condition of undertaking such research that all materials that have come into contact with biological materials be decontaminated before disposal to comply with Biohazard guidelines. This is a further function of the autoclave, and without this item of equipment such compliance is not possible, and the research must terminate. This item of equipment supports a large number of HQP within 2 very active Research Groups contributing to Bioengineering discoveries in Canada.

我们正在申请资金用于蒸汽灭菌器（或高压灭菌器），以支持Andrew Daugulis（AD）和Kimberly Woodhouse（KW）博士的研究，他们都与生物系统一起工作，需要该设备提供的无菌条件。该消毒器旨在取代一个现在已经超过10年的，需要定期和昂贵的维护。AD培养微生物用于生产高价值的营养品，以及用于有毒环境污染物的生物修复。在所有情况下，为了确保没有感染微生物的污染，所有材料（玻璃器皿、培养容器、生长培养基）必须在使用前灭菌，这是高压灭菌器的目的。KW从事的研究涉及在组织工程和再生医学应用中模仿自然的材料生产。 这些分子是在动脉和其他组织中发现的提供弹性的人类蛋白质弹性蛋白的片段。 KW使用人类弹性蛋白基因的一部分，将其插入细菌的DNA中，然后在培养容器中生长。 这项工作需要绝对无菌的材料，再次提供使用高压灭菌器。此外，在共用研究设施的两个研究小组之间，野生微生物菌株（AD）和大肠杆菌细胞（KW）的转基因菌株之间不会出现任何交叉污染，这将通过使用高压灭菌器来防止。最后，进行这种研究的一个条件是，所有与生物材料接触的材料在处置之前都必须经过净化，以符合生物危害准则。这是高压灭菌器的另一个功能，如果没有该设备，则不可能符合要求，研究必须终止。该设备支持2个非常活跃的研究小组内的大量HQP，为加拿大的生物工程发现做出贡献。