Regulation of contractility in the teleost heart

硬骨鱼心脏收缩力的调节

• 批准号: 2321-2009
• 负责人: Tibbits, Glen
• 金额: $ 3.5万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=513080
• 关键词: Regulation; contractility; teleost; heart

This NSERC program of research addresses the mechanisms by which the strength of the heart beat is regulated in fish. Although our primary interest is the salmonids which include rainbow trout, sockeye salmon and steelhead, other fish which function in warmer waters (e.g. zebrafish and tilapia) will be studied for an evolutionary perspective. The salmonids are of great importance socially, economically and strategically in Canada. How hearts from fish function is of great interest physiologically because their aquatic habitat exposes them to sudden and challenging changes in water ion concentrations (including Ca and Na), water temperature and pH that are not experienced by mammals in their terrestrial environments. Thus a major objective in this program of research is to understand how the environmental differences have dictated the molecular design of the hearts of these organisms. Another major objective is to understand how environmental changes (e.g. temperature and pH) acutely impact the basic regulatory mechanisms in the teleost heart on a molecular and cellular level, thereby impacting cardiac contractility. Cardiac contractility is quite variable but is regulated on a beat-to-beat basis by the amount of calcium (Ca) delivered to the contractile element and/or the sensitivity of the contractile element to Ca. Thus this program of research will focus on the structure and function of specific proteins in the cells that make up fish heart that regulate calcium delivery and removal and the contractile response to these signals. We will use techniques from bioinformatics, molecular biology, confocal microscopy, biophysics and physiology.

这个NSERC研究计划解决了鱼类心跳强度调节的机制。 虽然我们的主要兴趣是鲑鱼，包括虹鳟鱼，红鲑鱼和钢头，其他鱼类的功能，在温暖的沃茨（如斑马鱼和罗非鱼）将进行研究的进化角度。 鲑鱼在加拿大具有重要的社会、经济和战略意义。 鱼类心脏的功能在生理学上具有很大的意义，因为它们的水生栖息地使它们暴露于水离子浓度（包括Ca和Na），水温和pH值的突然和具有挑战性的变化，而哺乳动物在陆地环境中不会经历这些变化。因此，这项研究计划的一个主要目标是了解环境差异如何决定这些生物体心脏的分子设计。另一个主要目标是了解环境变化（例如温度和pH值）如何在分子和细胞水平上严重影响硬骨鱼心脏的基本调节机制，从而影响心脏收缩力。心脏收缩力是相当可变的，但在心跳到心跳的基础上通过递送到收缩元件的钙（Ca）的量和/或收缩元件对Ca的敏感性来调节。 因此，这项研究计划将集中在组成鱼心脏的细胞中特定蛋白质的结构和功能上，这些蛋白质调节钙的输送和去除以及对这些信号的收缩反应。 我们将使用生物信息学，分子生物学，共聚焦显微镜，生物物理学和生理学的技术。