Biosynthesis of plant extracellular lipids

植物细胞外脂质的生物合成

• 批准号: 327061-2011
• 负责人: Rowland, Owen
• 金额: $ 3.42万
• 依托单位: Carleton University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=514991
• 关键词: Biosynthesis; plant; extracellular; lipids

Agriculture worldwide will face many challenges over the coming decades, including those imposed by predicted changes in global climate patterns. In this context, a detailed understanding of plant adaptations that protect them against environmental stresses, such as drought, salt stress and pathogen attack, is critical for generating crop varieties that are more stress resistant. My NSERC Discovery research program is aimed at understanding the biosynthesis in plants of protective surface barriers that are composed of polymerized lipids and associated waxes. These chemically-related barriers are: 1) cuticle coating the aerial surfaces of plants, 2) suberin found in the cells walls of various external and internal tissue layers including roots, and 3) sporopollenin found in the outermost layer of the pollen spore coat. The coordinated activities of a large number of enzymes are required to produce the variety of fatty acid derivatives that form these surface lipid matrices. Over the next five years, my research team will characterize three classes of enzymes producing the following surface lipid molecules: 1) fatty alcohols, 2) free fatty acids, and 3) alkanes (fully reduced hydrocarbon molecules, similar to gasoline). We will also characterize the effects of mutating and over-expressing the genes encoding these enzymes on the ability of plants to withstand environmental stressors. This will give further insights into the structures and functions of plant surface lipid barriers. In addition to providing information and tools for the development of stress-tolerant plants, plant surface lipids are composed of renewable hydrocarbons that are chemically suitable for replacement of petroleum products as sources of energy and chemical feedstocks. Fundamental knowledge of plant surface lipid biosynthesis at the molecular level is critical for harnessing this renewable chemical resource to its full potential. Altogether, the proposed research will provide important fundamental knowledge of a set of plant lipid modification enzymes and insights into the ecophysiological functions of lipid-based surface barriers. In addition, the program will provide advanced training to students in plant molecular biotechnology.

未来几十年，全球农业将面临许多挑战，包括全球气候模式预测变化带来的挑战。在这种情况下，详细了解植物的适应性，保护他们免受环境压力，如干旱，盐胁迫和病原体的攻击，是至关重要的，以产生作物品种，更耐胁迫。我的NSERC发现研究计划旨在了解植物中由聚合脂质和相关蜡组成的保护性表面屏障的生物合成。这些与化学有关的屏障是：1）覆盖植物气生表面的角质层，2）在包括根在内的各种外部和内部组织层的细胞壁中发现的木栓质，和3）在花粉孢子衣的最外层中发现的孢粉素。需要大量酶的协调活性来产生形成这些表面脂质基质的各种脂肪酸衍生物。在接下来的五年里，我的研究团队将描述三种产生以下表面脂质分子的酶：1）脂肪醇，2）游离脂肪酸和3）烷烃（完全还原的烃分子，类似于汽油）。我们还将表征突变和过度表达编码这些酶的基因对植物承受环境压力的能力的影响。这将有助于进一步了解植物表面脂质屏障的结构和功能。除了为耐胁迫植物的发展提供信息和工具外，植物表面脂质还由可再生碳氢化合物组成，这些碳氢化合物在化学上适合替代石油产品作为能源和化学原料。在分子水平上了解植物表面脂质生物合成的基础知识对于充分利用这种可再生化学资源至关重要。总而言之，拟议的研究将提供一组植物脂质修饰酶的重要基础知识和深入了解基于脂质的表面屏障的生理生态功能。此外，该计划将为学生提供植物分子生物技术方面的高级培训。