Hydrophobins as scaffolds for designer enzyme complexes and catalytic surfaces

疏水蛋白作为设计酶复合物和催化表面的支架

• 批准号: 405730-2011
• 负责人: Gandier, JulieAnne
• 金额: $ 3.64万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Vanier Canada Graduate Scholarships - Doctoral
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=515589
• 关键词: Hydrophobins; scaffolds; designer; enzyme; complexes

Hydrophobins are small proteins produced by fungi of the ascomycete and basidiomycete divisions for adherence to a solid surface or emergence of a liquid phase. Of particular interest are proteins belonging to class I of the hydrophobin family. They are unique in their ability to spontaneously form membranes at interfaces (e.g. solid/water, water/air) and aggregates in solution. The aim of this research is to learn how to control the ability of EAS, a class I hydrophobin, to self-assemble in order to create macromolecules able to catalyze multiple reactions. Determining how aggregation is regulated will also benefit biomedical research. Hydrophobin aggregate structure is reminiscent of amyloid fibres associated with Alzheimer's disease and type II diabetes.

疏水蛋白是由子囊菌和担子菌分裂的真菌产生的小蛋白质，用于附着在固体表面或出现液相。特别令人感兴趣的是属于疏水蛋白家族第一类的蛋白质。它们在界面（如固体/水、水/空气）自发形成膜和在溶液中聚集的能力是独一无二的。本研究的目的是学习如何控制一类疏水蛋白EAS的自组装能力，从而产生能够催化多种反应的大分子。确定聚集是如何被调控的也将有利于生物医学研究。疏水蛋白聚集结构使人联想到与阿尔茨海默病和II型糖尿病相关的淀粉样纤维。