Optimization of rhamnolipid production for industrial use through genetic selection and manipulation of a rhamnolipid-producing bacterial species: an integrated approach.

通过遗传选择和操作鼠李糖脂生产细菌物种来优化工业用途的鼠李糖脂生产：一种综合方法。

• 批准号: 405731-2011
• 负责人: Saleh, AhmadMohammadAbdelmawgoud
• 金额: $ 3.64万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Vanier Canada Graduate Scholarships - Doctoral
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=515590
• 关键词: Optimization; rhamnolipid; production; industrial; use

Rhamnolipids (RLs) are bacterially produced substances having detergent character; thus they are also called biodetergents. RLs are growingly considered as potential biotechnological molecules for their recently-discovered wide spectrum of applications. They find applications as petroleum recovery agents, as antifungal for crop protection, in cosmetic and hygienic preparations, as emulsifying agents and for treatment of skin burns and ulcers. Besides, they have antimicrobial and promising anticancer activities. RLs are sources of fine chemicals like rhamnose sugar, and act as dispersing agents. Interestingly, RLs have applications in the field of nanobiotechnology; acting as effective dispersing agent for preparation of gold, silver and nickel nanoparticles. The interest in RLs has become even more urging than ever especially for bioremediation purposes because of their high efficiencies in cleaning-up hydrocarbon-contaminated marine and soil environments. In such situations, RLs serve as the low toxicity, and as the environmentally-friendly alternatives to synthetic surfactants. This wide scope of RLs applications has elicited the need for an industrial-level production, yet their production costs are impractically high due to their low bacterial yields. Improvements of RLs bacterial yields using classical methods of optimization of fermentation conditions and of culture medium components were not satisfactory. Hence, the focus of this study is to implement the advanced bioengineering techniques to genetically improve RL-producing strain. This is the most effective and feasible approach to increase RL yields to cost-effective levels. This project is going to put in hand a green alternative to the tons of noxious synthetic detergents consumed daily and harming Canadian environments. Moreover, it will provide Canada with an effective, environmentally-friendly, safe solution protecting Canadian environments against the disastrous consequences of accidental oil spills.

鼠李糖脂 (RL) 是细菌产生的具有洗涤剂特性的物质；因此它们也被称为生物洗涤剂。由于最近发现的广泛应用，RL 越来越被认为是潜在的生物技术分子。它们可用作石油回收剂、农作物保护的抗真菌剂、化妆品和卫生制剂、乳化剂以及治疗皮肤烧伤和溃疡。此外，它们还具有抗菌和有前景的抗癌活性。 RL 是鼠李糖等精细化学品的来源，并充当分散剂。有趣的是，RL 在纳米生物技术领域有应用；作为制备金、银、镍纳米粒子的有效分散剂。人们对 RL 的兴趣比以往任何时候都更加强烈，特别是在生物修复方面，因为它们在清理受碳氢化合物污染的海洋和土壤环境方面具有很高的效率。在这种情况下，RL 可以作为合成表面活性剂的低毒且环保的替代品。 RL 的广泛应用引发了对工业级生产的需求，但由于其细菌产量低，其生产成本高得不切实际。使用优化发酵条件和培养基成分的经典方法来提高 RL 细菌产量并不令人满意。因此，本研究的重点是实施先进的生物工程技术来遗传改良 RL 生产菌株。这是将 RL 产量提高到具有成本效益水平的最有效、最可行的方法。该项目将提供一种绿色替代品，替代每天消耗的大量有毒合成洗涤剂，并损害加拿大的环境。此外，它将为加拿大提供有效、环保、安全的解决方案，保护加拿大环境免受意外石油泄漏的灾难性后果。