Dendrimers in gene delivery: structural analysis of dendrimer complexes with DNA and RNA by AFM, affinity capillary electrophoresis, FTIR, CD and fluorescence spectroscopic methods

基因传递中的树枝状聚合物：通过 AFM、亲和毛细管电泳、FTIR、CD 和荧光光谱方法对树枝状聚合物与 DNA 和 RNA 的复合物进行结构分析

• 批准号: 89733-2010
• 负责人: TajmirRiahi, HeidarAli
• 金额: $ 2.19万
• 依托单位: Université du Québec à Trois-Rivières
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=521837
• 关键词: Dendrimers; gene; delivery; structural; analysis

Among polymers, dendrimers are unique synthetic macromolecules of nanometer dimensions with highly branched structure and globular shape capable of transporting DNA, RNA and drug with high efficiency. This research proposal is designed to study the structural organization of dendrimer complexes with DNA and RNA, using laser induced fluorescence capillary electrophoresis, FTIR, CD spectroscopic methods and atomic force microscopy (AFM). In recent reports from our laboratory, we have analysed the structural organizations of cationic lipid complexes with DNA and RNA and examined their potential applications in gene delivery (1, 2). In a constant search for designing tools to deliver gene in vivo, a variety of synthetic polymers have been tested and used. The most common polyamidoamide dendrimers (PAMAM)) are positively charged at physiological pH and strongly react with DNA and RNA.

在聚合物中，树枝状聚合物是具有高度支化结构和球形形状的独特的纳米尺寸的合成大分子，能够高效地运输DNA、RNA和药物。本研究采用激光诱导荧光毛细管电泳、傅立叶变换红外光谱、圆二色光谱和原子力显微镜（AFM）等方法，对树枝状大分子与DNA和RNA复合物的结构进行了研究。在我们实验室最近的报告中，我们分析了阳离子脂质复合物与DNA和RNA的结构组织，并研究了它们在基因递送中的潜在应用（1，2）。在不断寻找设计工具以在体内递送基因的过程中，已经测试和使用了各种合成聚合物。最常见的聚酰胺酰胺树状聚合物（PAMAM）在生理pH下带正电荷，并与DNA和RNA强烈反应。