Physiology and toxicology of cellular ion fluxes in plant roots

植物根部细胞离子通量的生理学和毒理学

• 批准号: 217277-2009
• 负责人: Kronzucker, Herbert
• 金额: $ 5.17万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=523746
• 关键词: Physiology; toxicology; cellular; ion; fluxes

Our laboratory studies the physiological ecology of nutrient acquisition in terrestrial plants. It examines the role of nutrient ion fluxes at cellular, whole-organism, and ecosystem levels, in several key areas: nutrition and yield potential in cereals, forest succession, cellular ionic interactions, nutrient ion toxicities, and salt stress. The current lead foci are the acquisition mechanisms for the macronutrients nitrogen and potassium, mechanisms for the toxic entry of sodium, and the energy cost of flux mechanisms at the plasma membrane of root cells. The experimental approach draws heavily upon recent molecular discoveries and ranges from the high-precision measurement of nutrient fluxes using radiotracers to the use of ion-selective electrodes and fluorescence dyes, ionic and metabolic profiling of tissues, respiration analysis, growth trials, and mathematical modeling of cellular flux processes. The laboratory is known for its investigation of the nutrient physiology of intact plants, the development of novel methods to tackle the complexities associated with whole systems, and its focus on species of agricultural and silvicultural importance.

本实验室主要研究陆生植物吸收养分的生理生态学。它研究了营养离子通量在细胞，整个生物体和生态系统水平上的作用，在几个关键领域：谷物的营养和产量潜力，森林演替，细胞离子相互作用，营养离子毒性和盐胁迫。目前的主要焦点是大量营养素氮和钾的获取机制，钠的毒性进入机制，以及根细胞质膜通量机制的能量成本。实验方法在很大程度上借鉴了最近的分子发现和范围从使用放射性示踪剂的营养通量的高精度测量到使用离子选择性电极和荧光染料，离子和组织的代谢分析，呼吸分析，生长试验和细胞通量过程的数学建模。该实验室以研究完整植物的营养生理学，开发新方法来解决与整个系统相关的复杂性，以及专注于农业和造林重要性物种而闻名。