Supplement for Role of Environmental Weathering and Gastrointestinal Digestion on the Bioavailability and Toxicity of Microplastic and Cadmium Mixtures

《多样性补充资料》项目摘要 在本增刊中，博士后候选人贾斯汀·斯科特博士将与整体 了解有机化合物在风化的MPS和纳米塑料(NPs)上的吸附，以及 MPS和NPs在鱼类(RTgutGC)和人(Caco 2)肠道细胞系中的生物积累和毒性。这将是 使我们能够更好地了解它们在摄入后的转化和命运，并确定暴露的风险和 潜在的不利影响。贾斯汀将通过学习高级光谱学来补充他的毒理学培训 和环境化学方法，在豪尔赫·冈萨雷斯·埃斯特雷拉博士的监督下。通过 结合了动态光散射(DLS)、Zeta电位、傅里叶变换等分析技术 红外光谱(FTIR)、扫描电子显微镜(SEM)和裂解气相色谱质谱(DGC) 分光光度法(Py-GC-MS)我们将更好地了解MP和NPS的反应性和不良反应 效果。重要的是，特定风化(即，紫外线老化和氧化)的MPS/NPS类型的反应性(即， 聚乙烯、聚氯乙烯和聚丙烯)与特定的有机化学混合物(即，林丹， 将测定二氯二苯基二氯乙烯(DDE)和嘧菌酯)。此外，它的不利影响 MPS/NPs单独暴露或与有机物联合暴露时，将使用与生理相关的暴露进行评估 该系统包括体外消化和肠道细胞系。我们假设环境 MPS和NPS的风化会增加有机化学混合物在其上的吸附，这将增加 肠道上皮对污染物的摄取。此外，胃肠道和消化过程将 允许从MPS和NPS中解吸化学物质，并增加生物积累和细胞毒性。目标1 评估风化MP和NP与曝光介质中的有机化学混合物的反应性；目的2： 评估有机化学品混合物和MPS/NPs在暴露系统中的去向；以及目标3：评估 胃肠道消化过程对MPS/NPS与有机化学品反应的影响 肠道细胞。

Project Summary of Diversity Supplement In this supplement, the postdoctoral fellow candidate Dr. Justin Scott will carry out a project with the overall goal of understanding the sorption of organic compounds onto weathered MPs and nanoplastics (NPs), and MPs and NPs bioaccumulation and toxicity in fish (RTgutGC) and human (Caco2) intestinal cell lines. This will allow us to better understand their transformation and fate after ingestion and determine exposure risk and potential adverse effects. Justin will complement his toxicological training by learning advanced spectroscopy and environmental chemistry methodologies under the supervision of Dr. Jorge Gonzalez Estrella. Through the incorporation of analytical techniques such as dynamic light scattering (DLS), zeta potential, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and pyrolysis gas chromatography mass spectrophotometry (py-GC-MS) we will gain a better understanding of MP and NPs reactivity and adverse effects. Importantly, the reactivity of specific weathered (i.e., UV aged and oxidized) MPs/NPs types (i.e., polyethylene, polyvinyl chloride, and polypropylene) with specific organic chemical mixtures (i.e., lindane, dichlorodiphenyldichloroethylene (DDE), and azoxystrobin) will be determined. In addition, the adverse effect of MPs/NPs alone or in co-exposure with organics will be evaluated using a physiologically relevant exposure system which includes in vitro digestion and intestinal cells lines. We hypothesize that environmental weathering of MPs and NPs will increase sorption of organic chemical mixtures onto them, which will increase the contaminant uptake by the intestinal epithelium. Moreover, gastrointestinal and digestive processes will allow desorption of chemicals from MPs and NPs and an increase in bioaccumulation and cytotoxicity. Aim .1 Evaluate the reactivity of weathered MP and NP with organic chemical mixtures in exposure media; Aim 2: Evaluate the Fate of organic chemical mixtures and MPs/NPs in the exposure system; and Aim 3: Evaluate the role of gastrointestinal digestion processes on MPs/NPs reactivity with organic chemicals and effect in intestinal cells.