Supplement for Role of Environmental Weathering and Gastrointestinal Digestion on the Bioavailability and Toxicity of Microplastic and Cadmium Mixtures

补充环境风化和胃肠消化对微塑料和镉混合物的生物利用度和毒性的作用

• 批准号: 10854398
• 负责人: Jorge Gonzalez Estrella
• 金额: $ 22.04万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-15 至 2025-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10854398
• 关键词: Address; Adverse effects; Affect; Affinity; Attention; Binding; Biological Availability; Caco-2 Cells; Cadmium; Cell Line; Cells; Cellular biology; Chemicals; Chemistry; Collaborations; Communities; Complement; Consumption; Data; Digestion; Dose; Ecosystem; Endocytosis; Endosomes; Environment; Environmental Engineering technology; Environmental Pollutants; Fishes; Fourier Transform; Fresh Water; Gas Chromatography; Gastrointestinal Process; Goals; Graduate Degree; Health; Health Hazards; Heavy Metals; Homeostasis; Human; In Vitro; Ingestion; Intestines; Knowledge; Learning; Lindane; Link; Liquid substance; Lysosomes; Mass Chromatography; Metabolism; Metals; Methodology; Mineral Waters; Modification; Oklahoma; Organic Chemicals; Organism; Physiological; Physiology; Plastics; Polyethylenes; Polypropylenes; Polyvinyl Chloride; Postdoctoral Fellow; Process; Reporting; Research; Research Personnel; Risk; Role; Route; Scanning Electron Microscopy; Scientist; Specific qualifier value; Spectrophotometry; Spectrum Analysis; Students; Supervision; Surface; System; Techniques; Testing; Toxic effect; Toxicology; Trace metal; Training; Weather; World Health Organization; acute toxicity; aged; aquatic organism; aqueous; bioaccumulation; career; cellular imaging; cytotoxicity; design; environmental chemical; environmental chemistry; environmental toxicology; gastrointestinal; graduate student; innovation; intestinal epithelium; light scattering; metal metabolism; meter; novel; parent grant; pollutant; skills; student training; theories; tool; ultraviolet; undergraduate student; uptake; zeta potential

Project Summary of Diversity Supplement In this supplement, the postdoctoral fellow candidate Dr. Justin Scott will carry out a project with the overall goal of understanding the sorption of organic compounds onto weathered MPs and nanoplastics (NPs), and MPs and NPs bioaccumulation and toxicity in fish (RTgutGC) and human (Caco2) intestinal cell lines. This will allow us to better understand their transformation and fate after ingestion and determine exposure risk and potential adverse effects. Justin will complement his toxicological training by learning advanced spectroscopy and environmental chemistry methodologies under the supervision of Dr. Jorge Gonzalez Estrella. Through the incorporation of analytical techniques such as dynamic light scattering (DLS), zeta potential, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and pyrolysis gas chromatography mass spectrophotometry (py-GC-MS) we will gain a better understanding of MP and NPs reactivity and adverse effects. Importantly, the reactivity of specific weathered (i.e., UV aged and oxidized) MPs/NPs types (i.e., polyethylene, polyvinyl chloride, and polypropylene) with specific organic chemical mixtures (i.e., lindane, dichlorodiphenyldichloroethylene (DDE), and azoxystrobin) will be determined. In addition, the adverse effect of MPs/NPs alone or in co-exposure with organics will be evaluated using a physiologically relevant exposure system which includes in vitro digestion and intestinal cells lines. We hypothesize that environmental weathering of MPs and NPs will increase sorption of organic chemical mixtures onto them, which will increase the contaminant uptake by the intestinal epithelium. Moreover, gastrointestinal and digestive processes will allow desorption of chemicals from MPs and NPs and an increase in bioaccumulation and cytotoxicity. Aim .1 Evaluate the reactivity of weathered MP and NP with organic chemical mixtures in exposure media; Aim 2: Evaluate the Fate of organic chemical mixtures and MPs/NPs in the exposure system; and Aim 3: Evaluate the role of gastrointestinal digestion processes on MPs/NPs reactivity with organic chemicals and effect in intestinal cells.

多样性补充项目总结 在本增刊中，博士后研究员候选人贾斯汀·斯科特博士将与整体一起开展一个项目 目标是了解有机化合物对风化MP和纳米塑料（NP）的吸附，以及 MP和NP在鱼（RTgutGC）和人（Caco 2）肠细胞系中的生物累积和毒性。这将 使我们能够更好地了解它们在摄入后的转化和命运，并确定暴露风险， 潜在的不利影响。贾斯汀将通过学习先进的光谱学来补充他的毒理学培训 在豪尔赫·冈萨雷斯·埃斯特雷拉博士的监督下，通过 结合分析技术，如动态光散射（DLS）、ζ电位、傅里叶变换 红外光谱（FTIR）、扫描电子显微镜（SEM）和热解气相色谱质谱 通过分光光度法（py-GC-MS），我们将更好地了解MP和NPs的反应性和不良反应。 方面的影响.重要的是，特定风化的反应性（即，UV老化和氧化的）MP/NP类型（即， 聚乙烯，聚氯乙烯，和聚丙烯）与特定的有机化学混合物（即，亚麻布， 二氯二苯二氯乙烯（DDE）和嘧菌酯）。此外，不利影响 单独或与有机物共同暴露的MP/NP将使用生理相关暴露进行评价 该系统包括体外消化和肠细胞系。我们假设环境 MP和NP的风化将增加有机化学混合物在其上的吸附，这将增加 肠上皮细胞对污染物的吸收。此外，胃肠和消化过程将 允许化学品从MP和NP中解吸，并增加生物累积和细胞毒性。目标.1 评价风化MP和NP与暴露介质中有机化学混合物的反应性;目标2： 评估有机化学混合物和MP/NP在暴露系统中的归宿;以及目标3： 胃肠道消化过程对MP/NPs与有机化学品反应性的作用以及对 肠细胞