Factors affecting the concentration of health-beneficial compounds in soybean

影响大豆中有益健康化合物浓度的因素

• 批准号: 238272-2010
• 负责人: Seguin, Philippe
• 金额: $ 1.97万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=524270
• 关键词: Factors; affecting; concentration; health; beneficial

Soybean [Glycine max (L.) Merr.] contains high concentrations of isoflavone and tocopherol, which both have putative health-beneficial properties. The North American market value for soybean isoflavones alone is of 500 million $. The proposed research program aims at providing an understanding of the factors affecting the concentrations of isoflavone and tocopherol in soybean. Using studies conducted in multiple environments we will identify among a wide range of abiotic factors (i.e., air and soil temperature, soil moisture, fertility, etc...) those affecting the most soybean isoflavone and tocopherol concentrations. In controlled environments, we will also determine if particular growth stages are more responsive to specific abiotic factors, focusing on the impact of air temperature and soil moisture, which were previously reported to significantly affect isoflavone and tocopherol concentrations in soybean. In these later studies we will look at the impact of several stress treatments on soybean isoflavone and tocopherol concentrations using HPLC, as well as gene expression. We will use real-time QRT-PCR technology to quantify transcript levels of genes involved in isoflavone synthesis [phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone reductase (CHR), isoflavone synthase (IFS), malonyl transferase (MT), and glucosyl transferase (GT)] and tocopherol synthesis [homogentisate phytyltransferase (HPT), 7,2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ MT), tocopherol cyclase (TC), and gamma-tocopherol methyltransferase (gamma-TMT)] in different plant tissues throughout soybean development, following different abiotic stress treatments. In order to determine how stress affects soybean isoflavone and tocopherol concentrations, we will study their relation to genes expression. Ultimately, this research will contribute to the development of new value-added productions and create economic opportunities for Canadian agricultural producers and associated agri-industries.

大豆[Glyine max(L.)[MERR.]含有高浓度的异黄酮和生育酚，这两种物质都具有公认的有益健康的特性。仅大豆异黄酮在北美的市场价值就达5亿美元。这项拟议的研究计划旨在了解影响大豆中异黄酮类和生育酚含量的因素。利用在多个环境中进行的研究，我们将在广泛的非生物因素中确定(即，空气和土壤温度、土壤湿度、肥力等)。那些对大豆异黄酮和生育酚浓度影响最大的。在受控环境中，我们还将确定特定的生长阶段是否对特定的非生物因素更敏感，重点是气温和土壤湿度的影响，此前有报道称，这两种因素会显著影响大豆中异黄酮类和生育酚的含量。在后面的这些研究中，我们将使用高效液相色谱法来研究几种胁迫处理对大豆异黄酮类和生育酚含量以及基因表达的影响。我们将利用实时荧光定量聚合酶链式反应技术对大豆不同发育过程中异黄酮合成相关基因[苯丙氨酸解氨酶(PAL)、查尔酮合成酶(CHS)、查尔酮还原酶(CHR)、异黄酮合酶(IFS)、丙二酰基转移酶(MT)和葡萄糖基转移酶(GT)]和生育酚合成相关基因[同源植酸植基转移酶(HPT)、7，2-甲基-6-植基-1，4-苯二酚甲基转移酶(MPBMT)、生育酚环化酶(TC)和γ-生育酚甲基转移酶(Gamma-TMT)]的转录水平进行定量研究。为了确定胁迫如何影响大豆异黄酮和生育酚浓度，我们将研究它们与基因表达的关系。最终，这项研究将有助于开发新的附加值产品，并为加拿大农业生产者和相关农业产业创造经济机会。