A quantitative look at the motion of proteins in cells

定量观察细胞中蛋白质的运动

• 批准号: 250205-2010
• 负责人: Fradin, Cecile
• 金额: $ 4.66万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=524958
• 关键词: quantitative; look; motion; proteins; cells

Because living systems are highly dynamics, understanding cellular processes, that is understanding how a cell is working, will require studying and understanding protein motions. Here we are interested in the interplay between protein dynamics, that is how a protein might move across the cell through diffusion and interactions with other molecules, and the establishment of spatial concentration gradients, that is how this protein might end up being concentrated in certain areas of the cell. We propose to apply a range of fluorescence techniques adapted to the detect and quantification of protein localization and dynamics to answer two different questions, each about a cellular system where protein concentration gradients play a major role.

由于生命系统是高度动态的，了解细胞过程，即了解细胞是如何工作的，将需要研究和了解蛋白质运动。在这里，我们感兴趣的是蛋白质动力学之间的相互作用，即蛋白质如何通过扩散和与其他分子的相互作用在细胞内移动，以及空间浓度梯度的建立，这就是这种蛋白质可能最终集中在细胞的特定区域的原因。我们建议应用一系列适合于检测和量化蛋白质定位和动力学的荧光技术来回答两个不同的问题，每个问题都是关于蛋白质浓度梯度发挥主要作用的细胞系统。