A quantitative look at the motion of proteins in cells

因为生命系统是高度动态的，理解细胞过程，也就是理解细胞是如何工作的，将需要研究和理解蛋白质的运动。这里我们感兴趣的是蛋白质动力学之间的相互作用，即蛋白质如何通过扩散和与其他分子的相互作用在细胞中移动，以及空间浓度梯度的建立，即蛋白质如何最终集中在细胞的特定区域。我们建议应用一系列适合于检测和定量蛋白质定位和动力学的荧光技术来回答两个不同的问题，每个问题都是关于蛋白质浓度梯度起主要作用的细胞系统。

Because living systems are highly dynamics, understanding cellular processes, that is understanding how a cell is working, will require studying and understanding protein motions. Here we are interested in the interplay between protein dynamics, that is how a protein might move across the cell through diffusion and interactions with other molecules, and the establishment of spatial concentration gradients, that is how this protein might end up being concentrated in certain areas of the cell. We propose to apply a range of fluorescence techniques adapted to the detect and quantification of protein localization and dynamics to answer two different questions, each about a cellular system where protein concentration gradients play a major role.