"Three genomes, two organisms, one host: Interactions between the bacterium Wolbachia pipientis, its WO bacteriophage and Drosophila"

“三个基因组、两种生物体、一种宿主：Wolbachia pipientis 细菌、其 WO 噬菌体和果蝇之间的相互作用”

• 批准号: 283196-2012
• 负责人: Harris, Harriet
• 金额: $ 2.04万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=525632
• 关键词: Three; genomes; two; organisms; host

Wolbachia pipientis are proteobacteria that have maintained symbiotic associations with host insects for millions of years. These bacteria exist within the cytoplasm of host cells, preferentially cells of the ovaries and testes, and are therefore endosymbionts. There are several intriguing aspects about this relationship between Wolbachia and flies which are currently being examined in my laboratory. First, the host does not seem to recognize the presence of Wolbachia because it does not mount a typical anti-bacterial immune response. My laboratory has been studying the mechanisms that enable these bacteria to survive and replicate inside host cells, and we have shown that the host and bacteria cooperate to reduce potential cellular and DNA damage caused by reactive oxygen species (ROS). ROS are generated at high levels in infected cells, and both host and bacterial antioxidant enzymes are recruited to neutralize them. This is the first time that cooperation of this type has been described between animal cells and bacteria.

Wolbachia pipientis是与宿主昆虫保持共生关系数百万年的变形菌。这些细菌存在于宿主细胞的细胞质中，优选卵巢和睾丸的细胞，因此是内共生体。关于沃尔巴克氏体和苍蝇之间的关系，有几个有趣的方面，目前正在我的实验室进行研究。首先，宿主似乎没有识别出沃尔巴克氏体的存在，因为它没有产生典型的抗细菌免疫反应。我的实验室一直在研究使这些细菌能够在宿主细胞内生存和复制的机制，我们已经证明宿主和细菌合作减少活性氧（ROS）引起的潜在细胞和DNA损伤。ROS在受感染的细胞中以高水平产生，宿主和细菌的抗氧化酶都被招募来中和它们。这是第一次在动物细胞和细菌之间描述这种类型的合作。