Post Translational Addition of Amino Acids - Mechanisms Substrate Recognition

氨基酸的翻译后添加 - 底物识别机制

• 批准号: 341453-2012
• 负责人: Fahlman, Richard
• 金额: $ 1.89万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=527313
• 关键词: Post; Translational; Addition; Amino; Acids

Protein degradation is fundamental to a variety of cellular processes which include gene regulation, attenuating of proteolytic cascades and the removal of mis-folded or damaged proteins. We are investigating the evolutionary conserved degradation signal known as the N-degron, which is the signal for N-End Rule degradation in both eubacteria and eukaryotes. The N-end rule pathway is a ubiquitous process that targets proteins for degradation that possess a particular N-terminal amino acid. Aminoacyl-tRNA protein transferases catalyze the post-translational addition of destabilizing amino acids from aminoacyl-tRNAs to the N-termini of substrate proteins, targeting them for N-End rule degradation.

蛋白质降解是多种细胞过程的基础，包括基因调控、蛋白水解级联的减弱和错误折叠或受损蛋白质的去除。 我们正在研究被称为N-degron的进化保守降解信号，这是真细菌和真核生物中N末端规则降解的信号。N末端规则途径是一种普遍存在的过程，其靶向具有特定N末端氨基酸的蛋白质进行降解。 氨酰-tRNA蛋白转移酶催化来自氨酰-tRNA的不稳定氨基酸翻译后添加到底物蛋白的N-末端，靶向它们进行N-末端规则降解。